Preparation method of compound alisol A and application thereof causing renal toxicity

A technology of alisol and compounds, which is applied in the field of medicine and can solve problems such as limitations and high experimental costs

Inactive Publication Date: 2012-09-19
SHENYANG PHARMA UNIVERSITY
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Problems solved by technology

Although the academic circle has made some progress in the toxicity research of traditional Chinese medicine monomer compounds, due to the separation, structure identification and quan

Method used

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  • Preparation method of compound alisol A and application thereof causing renal toxicity
  • Preparation method of compound alisol A and application thereof causing renal toxicity
  • Preparation method of compound alisol A and application thereof causing renal toxicity

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Experimental program
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Embodiment Construction

[0031] One, as the preparation method of 3 times of ethanol addition of known compound alisitol A

[0032] 1. Preparation of Alisma ethanol extract

[0033] Take Alisma, add 3 times of 80% ethanol (that is, the weight ratio of Alisma and ethanol is 1:3), cold soak for 12 h, heat and reflux for 5 h, filter, repeat 3 times, combine the filtrate, and pass through a rotary evaporator for 50 The solvent was evaporated to dryness at ℃ to obtain the extract of Alisma 80% ethanol extract.

[0034] 2. Preparation of Chloroform Extracts

[0035] Suspend the concentrated extract in water, and extract with petroleum ether, chloroform, ethyl acetate, n-butanol and other volumes successively, each 3 times. The chloroform extracts were combined, and the solvent was evaporated to dryness by a rotary evaporator at 50° C. to obtain the chloroform layer extract.

[0036] 3. Purification of compound alisol A

[0037]Take the chloroform extract, go through silica gel column chromatography (200...

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Abstract

The invention provides a preparation method of compound alisol A causing renal toxicity and an experimental method of the compound alisol A, aiming at solving the problem that the modern clinical practice and the pharmacology experiment are only limited to the description of the intoxicating phenomenon and are undefined in the material basis of the renal toxicity causing effect. The invention has the key points of: (1) preparing rhizoma alismatis ethanol extracting solution; (2) preparing chloroform extractant; (3) purifying compound alisol A; (4) detecting the proliferate inhibiting effect of the alisol A to the HKC (renal tubular epithelial cell) by an MTT (microwave theory and techniques) method; and (5) observing cytomorphology change by the Hoechst33342-PI fluorescent double staining method. The invention has the benefit effects that the basis is provided for the reasonable application of the traditional Chinese medicine rhizoma alismatis and the prevention and cure of the causing of the renal injury.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a preparation method of alisol A, a compound with nephrotoxic effect, and an application of alisol A to human kidney tubular epithelial cell line (HKC) in vitro cytotoxicity. Background technique [0002] Alisma is a plant in the family Alismaceae Alisma orientalis (Sam.) Juzep . of dry tubers. First published in "God [0003] "Nong Ben Cao Jing", all previous editions of "Chinese Pharmacopoeia" are recorded. In addition to being used as a traditional Chinese medicine for diuresis and dampness, it is also used as a medicinal diet and weight-loss health care products, which allows them to be taken for a long time. Although Alisma has been widely used clinically in recent years, people only pay attention to its therapeutic efficacy, but ignore its toxic effect on the kidneys after long-term use or excessive use, leaving hidden dangers for safe clinical use. Modern clinical prac...

Claims

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Application Information

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IPC IPC(8): C07J13/00G01N21/31G01N21/64
Inventor 陈晓辉毕开顺于治国俞悦马超耿璐璐
Owner SHENYANG PHARMA UNIVERSITY
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