Reagent composition for immunochromatography

An immunochromatography and composition technology, which is used in analytical materials, biological tests, material testing products, etc., can solve the problems that non-specific reactions cannot be fully inhibited and non-specific reactions cannot be inhibited, and the sensitivity will not be reduced or inhibited. Effects of nonspecific reactions

Inactive Publication Date: 2012-10-03
TANAKA PRECIOUS METAL IND
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Problems solved by technology

[0009] However, as surfactants used for suppressing coloration of the back plate or blank coloration, or for suppressing side reactions based on biological affinity or suppressing non-specific reactions, nonionic surfactants ( Such as Tween 20, Triton X-100, etc.), ionic surfactants or polyanions did not fully achieve the purpose, and there is also the problem of not being able to suppress non-specific reactions
[0010] The inventor of the present invention focused on the above-mentioned nonionic surfactants (such as Tween20, Triton X-100, etc.), ionic surfactants or polyanions, especially focused on nonionic surfactants and carried out experiments, and the results obtained found that , when the detection object in the sample is detected by immunochromatography, the occurrence of non-specific reactions will still be observed, and the problem that the non-specific reactions cannot be fully suppressed still exists

Method used

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  • Reagent composition for immunochromatography
  • Reagent composition for immunochromatography
  • Reagent composition for immunochromatography

Examples

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Embodiment

[0079] The following examples illustrate the effectiveness of the present invention, but the present invention is not limited thereto.

[0080] 1. Preparation of the determination part on the chromatography medium

[0081] A 25×2.5 cm cellulose membrane (manufactured by Millipore, trade name: HF120) was coated with a phosphate buffer (pH 7.4) diluted with 5 wt % isopropanol using an antibody coater (manufactured by BioDot). The diluted hCG polyclonal antibody (α-hCGα) at ​​a concentration of 1.0 mg / ml was dried to prepare a determination part on a chromatography medium.

[0082] 2. Preparation of Labeling Reagent Solution

[0083] To 0.5 ml of colloidal gold suspension (manufactured by Tanaka Precious Metals Co., Ltd., 80 nm), 0.1 ml of phosphate buffer (pH 7.4) and 0.1 ml of anti-hCG monoclonal antibody (α-hCGβ) diluted with phosphate buffer were added , let stand for 10 minutes at room temperature. Next, 0.1 ml of 10 wt % bovine serum albumin (BSA) diluted with phosphate ...

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Abstract

Provided are a sample diluent or a reagent composition for immunochromatography, wherein it is possible to inhibit nonspecific reactions compared to prior art in which an object to be detected was detected using an immunochromatography device. Also provided are an immunochromatography device which uses said reagent composition or diluent and which exerts high performance and sensitivity, and a detection kit which uses said reagent composition or diluent and with which it is possible to swiftly and easily perform examinations. A reagent composition for immunochromatography, a developer for immunochromatography, or a sample diluent for immunochromatography, which contain a buffer solution, a chelating agent, and a nonionic surfactant, are used when detecting an object to be detected in a sample by means of the immunochromatography method. As a consequence, it is possible to inhibit nonspecific reactions.

Description

technical field [0001] The present invention relates to a high-performance and high-sensitivity immunochromatographic reagent composition or immunochromatographic developing solution capable of suppressing nonspecific reactions. The present invention further relates to a detection kit and a detection method for rapidly and simply examining and measuring a detection target substance in a sample, which can suppress non-specific reactions. More specifically, the present invention relates to a detection kit that can perform detection quickly, easily and with high accuracy by directly supplying a detection sample without pretreatment to a sample adding portion of the detection kit. Background technique [0002] In recent years, as a strip immunoassay for immunization for immunochromatography that does not require pretreatment of a test sample, a simple in vitro diagnostic reagent that utilizes the specific reaction of an antibody to detect an antigen in a sample solution The imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543
CPCG01N33/558G01N33/76G01N33/54393G01N33/54388
Inventor 伊藤大辅芝井勇亮
Owner TANAKA PRECIOUS METAL IND
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