Application of gastrodiaelata blume parishin extractive in preparation of medicament for protecting brain

A technology of pericin and extract is applied to the application field of Gastrodia perixin extract in the preparation of brain protection medicine, and can solve the problem that there is no gastrodia elata extract and the like

Active Publication Date: 2012-10-17
北京协和制药二厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All of the above are the applications of Gastrodia elata in neurodegenerative diseases, but so far, there have been no reports on the application of Gastrodia elata extract in neuroprotection against irreversible brain damage caused by neurochemical reactions.

Method used

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  • Application of gastrodiaelata blume parishin extractive in preparation of medicament for protecting brain
  • Application of gastrodiaelata blume parishin extractive in preparation of medicament for protecting brain
  • Application of gastrodiaelata blume parishin extractive in preparation of medicament for protecting brain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Protective Effect of Perisin Extract of Tianma on Focal Cerebral Ischemia-Reperfusion Injury in Rats

[0027] 1. Experimental drugs

[0028] Gastrodia elata extract: prepared according to the preparation method described in the patent No. CN201110303629.X. Gastrodia elata extracts with 50% and 70% parisin derivative contents were selected as experimental drugs, respectively referred to as 50% Paresin derivatives, 70% paresin derivatives;

[0029] Perisin, Perisin B, and Perisin C monomers;

[0030] The above-mentioned paresin extract of Gastrodia elata and the monomers of paresin A, B, and C are suspended and dissolved in normal saline for later use;

[0031] Edaravone, purchased from Jiangsu Simcere Pharmaceutical Group;

[0032] Red tetrazolium (TTC), provided by Shanghai Bioengineering Company.

[0033] 2. Experimental method

[0034] Healthy SD rats, weighing 240-300g, SPF grade, male or female, were randomly divided into sham operation group, model c...

Embodiment 2

[0054] Example 2 The Protective Effect of Tianma Pelicin Extract on Rat Nerve Cell Damage Caused by N-Methyl D-Aspartic Acid

[0055] 1. Experimental materials

[0056] Gastrodia elata extract: prepared according to the preparation method described in the patent No. CN201110303629.X, using Gastrodia elata extracts with 50% and 70% parisin derivatives (respectively referred to as 50% parisin Derivative group, 70% paresin derivative group), diluted with normal saline to an appropriate concentration when used; polylysine is a product of sigma company; DMEM medium is a product of Gibco company; other reagents are of analytical grade.

[0057] N-Methyl D-Aspartic Acid (NMDA):

[0058] Experimental animals: 18 ordinary Sprague Dawley rats, weighing 250-280g, provided by the Experimental Animal Center of Shandong Provincial Natural Medicine Engineering Technology Research Center, certificate number: Ludongzhizi No. 20100515.

[0059] 2. Experimental method

[0060] 2.1 Primary cul...

Embodiment 3

[0081] Example 3 Protective effect of Tianma parisin extract on mice with blunt traumatic brain injury

[0082] 1. Experimental materials

[0083] Gastrodia elata extract: prepared according to the preparation method described in the patent No. CN201110303629.X, using Gastrodia elata extracts with 50% and 70% parisin derivatives (respectively referred to as 50% parisin derivative group, 70% parisin derivative group), diluted with normal saline to an appropriate concentration when used;

[0084] Automatic recorder for platform jumping experiment, Instrument and Electricity Department, Institute of Materia Medica, Chinese Academy of Medical Sciences; Fluorescence spectrophotometer, HITACHI Company, Japan; Microplate reader, Molecula, USA;

[0085] Experimental animals: clean Kunming mice, weighing 20-22g, provided by the Experimental Animal Center of Shandong Provincial Natural Medicine Engineering Skills Research Center, certificate number: Ludongzhizi No. 20100515.

[0086] ...

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Abstract

The invention relates to an application of gastrodiaelata blume parishin extractive in preparation of a medicament for protecting brain, wherein the medicament is used for treatment of brain injury. Total content of a parishin derivative in the gastrodiaelata blume parishin extractive is more than 50%, preferably more than 75%, more preferably more than 95%. The parishin provides a certain protection effects for cerebral ischemia reperfusion injury, nerve cell injury induced by N-methyl-D-aspartate and closed cerebral trauma. The extractive provided in the invention provides a new selection approach for developing the medicament for protecting brain.

Description

technical field [0001] The invention relates to the field of new applications of medicines, in particular to the application of Gastrodia elata parisin extract in preparing brain-protective medicines. Background technique [0002] Craniocerebral injury is a severe trauma caused by violence on the head, causing high mortality and disability. The mortality rate is between 4% and 7%, and the mortality rate of severe traumatic brain injury is as high as 50-60% %. Craniocerebral injury can be divided into closed injury and open injury. The former refers to the injury that the brain tissue does not communicate with the outside world; the latter refers to the injury that the brain tissue communicates with the outside world, with scalp and skull dehiscence, and cerebrospinal fluid or brain tissue overflow . [0003] Hundreds of thousands of people die from trauma every year in our country, and most of them die from fatal craniocerebral trauma. In order to improve the prognosis of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7034A61K36/8988A61P25/00
Inventor 王珂石建功赵立敏赵大龙邹德超张猛刘学军张秋佳
Owner 北京协和制药二厂有限公司
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