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Application of OsSRO1c gene in controlling rice drought resistance

A technology of drought resistance and genetics, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Inactive Publication Date: 2012-10-17
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the OsSRO1c gene is the downstream gene of the drought resistance transcription factor SNAC1, there is no relevant report on whether it can improve the stress resistance of rice.

Method used

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  • Application of OsSRO1c gene in controlling rice drought resistance
  • Application of OsSRO1c gene in controlling rice drought resistance
  • Application of OsSRO1c gene in controlling rice drought resistance

Examples

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Embodiment Construction

[0020] The following examples define the present invention and describe the method of the present invention in isolating OsSRO1c T-DNA insertion mutants, cloning a DNA fragment containing the complete coding segment of OsSRO1c gene, and verifying the function of OsSRO1c gene. According to all or part of the implementation steps described below, those skilled in the art can determine the essential features of the present invention, and without departing from the spirit and scope of the present invention, various changes and modifications can be made to the present invention, so that Different uses and conditions apply.

[0021] 1. Detection of rice endogenous OsSRO1c gene induced by stress

[0022] In order to preliminarily determine whether the OsSRO1c gene is related to stress resistance, the present invention firstly detected whether the rice endogenous gene OsSRO1c is induced by stress. The applicant selected the indica rice variety "Zhonghua 11" (ZH11 for short, a commerc...

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Abstract

The invention relates to the technical field of rice gene engineering. An application of OsSRO1c rice for raising drought durability to genetic improvement of rice drought resistance is obtained through separation, cloning and functional verification. OsSRO1c gene is cloned by using a method of sieving a T-DNA inserted rice mutant library, an expression level detection and an drought stress phenotype identification indicate that the mutant is coseparated with a drought sensitive phenotype; overexpression of OsSRO1c gene can enhance the drought durability of transgenic rice, thereby the functions and application approaches of the gene can be verified.

Description

technical field [0001] The invention relates to the field of rice genetic engineering. It specifically relates to the application of a rice OsSRO1c gene capable of improving drought tolerance through isolation, cloning and functional verification in the genetic improvement of rice drought resistance. The present invention uses a candidate gene screening method to clone the rice drought-resistant gene OsSRO1c, and the co-segregation test shows that the OsSRO1c mutant is closely related to the drought-sensitive phenotype, and the overexpression of the OsSRO1c gene can improve the drought-resistant ability of transgenic rice, confirming The function and application pathway of the gene. Background technique [0002] Plants will be affected by many environmental factors during the growth process. Drought, cold damage and high temperature will lead to large-scale crop yield reduction, which is the bottleneck of agricultural development in many areas. Cultivating stress-tolerant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
CPCC07K14/415C12N15/8273
Inventor 熊立仲游均
Owner HUAZHONG AGRI UNIV
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