Dyeing method for bacterial capsules in Haemophilus influenzae type b fermentation broth

Abstract

The invention discloses a dyeing method for bacterial capsules in Haemophilus influenzae type b fermentation broth. The method comprises the steps of pretreatment, smear preparation, smearing, drying, dyeing, secondary drying and microscopic examination The method employs the principle of negative dyeing, which guarantees that bacterial capsules are integral and thalluses occupy a great area and are easy to identify; a good dyeing effect is obtained, and color contrast between the capsules and a background is obvious, so the capsules can be easily observed; operation process is simple and does not have high requirements on operating skill of a tester, so the success rate of testing is high.

Description

technical field [0001] The invention relates to a bacterial staining method, in particular to a bacterial capsule staining method of Haemophilus influenzae type b fermentation liquid. Background technique [0002] Haemophilus influenzae (scientific name Haemophilus influenzae), referred to as Haemophilus, formerly known as Pfeiffer bacillus (or translation Bayfield bacteria) or influenza bacillus, belongs to the genus Haemophilus of the family Aliciaceae, is a non-motility Gram-negative bacilli. [0003] Haemophilus influenzae is classified into two types: encapsulated strains and non-encapsulated strains. Among them, the capsular Haemophilus influenzae type b, that is, Haemophilus influenzae type b (Hib for short) is one of the main causes of toxicity. In order to prevent invasive infection caused by Haemophilus influenzae, the Haemophilus influenzae type b conjugate vaccine made by covalently binding purified Hib capsular polysaccharide and tetanus toxoid protein is ofte...

AI Technical Summary

Problems solved by technology

First of all, the operation process is relatively complicated, which requires high operating skills of the experimenter, which may lead to the failure of the experiment; the stability of the dye is also high, and if a large particle dye is used, the observation result will often be affected; in addition, these After the method is stained, often only a few bacteria can be seen, and it needs to be searched in a large area under the microscope, which is very inconvenient for actual operation

Moreover, due to the high water content of the bacterial capsule, heating will cause the bacteria to lose water and shrink, and will separate from the dye around the cells to produce a transparent bright area, causing some non-capsulated bacteria to be mistaken for capsuled bacteria.

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