Effect testing method for duck hemorrhagic ovaritis inactivated vaccine

A technology for hemorrhagic oophoritis and inactivated vaccine, which is applied in the field of veterinary biological product inspection to achieve the effect of strong operability and objective method

Inactive Publication Date: 2012-10-17
INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are no detailed reports on objective, feasible, operable and scientific vaccine efficacy evaluation methods

Method used

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  • Effect testing method for duck hemorrhagic ovaritis inactivated vaccine
  • Effect testing method for duck hemorrhagic ovaritis inactivated vaccine
  • Effect testing method for duck hemorrhagic ovaritis inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Duck hemorrhagic oophoritis inactivated vaccine (ELISA antibody assay) efficacy test with the age of ducks, immunization dose, sampling time (1)

[0024] Use duck hemorrhagic ovariitis inactivated vaccine (batch number 201101, developed by Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences and Ruipu (Baoding) Biopharmaceutical Co., Ltd.) to immunize 10 shelduck ducks 560 days old and not infected with DHOV, 0.5ml / only, chest intramuscular injection. Two weeks after the first immunization, the second immunization was carried out according to the same dose and the same route. The serum of the ducks was collected 4 weeks after the second immunization, and the ELISA antibody detection method attached was used to detect the serum of the immune ducks. , the ELISA antibody positive rate of ducks in the immunized group was 80%, indicating that the vaccine efficacy test was qualified.

Embodiment 2

[0025] Example 2 Age of Ducks, Immunization Dose, and Sampling Time of Ducks Used in Efficacy Test of Duck Hemorrhagic Ovariitis Inactivated Vaccine (ELISA Antibody Assay) (2)

[0026] Use duck hemorrhagic ovariitis inactivated vaccine (batch number 201102, developed by Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry and Ruipu (Baoding) Biopharmaceutical Co., Ltd.) to immunize 10 Peking ducks that were 42 days old and not infected with DHOV, 1ml / Only, chest intramuscular injection. Two weeks after the first immunization, the same dose and the same route were used for the second immunization. The serum of the ducks was collected 4 weeks after the second immunization, and the serum of the immune ducks was detected by the ELISA antibody detection method attached. The ELISA antibody positive rate of ducks in the immunized group was 90%, indicating that the vaccine efficacy test was qualified.

Embodiment 3

[0027] Example 3 Duck age, immunization dose, sampling time (3)

[0028] Use duck hemorrhagic ovariitis inactivated vaccine (batch number 201103, developed by Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry and Ruipu (Baoding) Biopharmaceutical Co., Ltd.) to immunize 10 Peking ducks 180 days old and not infected with DHOV, 2ml / Only, chest intramuscular injection. Two weeks after the first immunization, the same dose and the same route were used for the second immunization. The serum of the ducks was collected 4 weeks after the second immunization, and the serum of the immune ducks was detected by the ELISA antibody detection method attached. The ELISA antibody positive rate of ducks in the immunized group was 100%, indicating that the vaccine efficacy test was qualified.

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Abstract

The present invention provides a serology effect testing method for a duck hemorrhagic ovaritis inactivated vaccine. According to the method, a duck hemorrhagic ovaritis inactivated vaccine is adopted to vaccinate ducks twice, the second time vaccination is performed after the first time vaccination is performed two weeks, duck serums are sampled to carry out ELISA antibody detection after the second time vaccination is performed 3-5 weeks, wherein the qualified standard of the vaccine effect test is that the ELISA antibody positive rate of the non-vaccinated group is 0% and the ELISA antibody positive rate of the vaccinated group is not less than 80%. The serology effect testing method for the duck hemorrhagic ovaritis inactivated vaccine in the present invention has characteristics of convenient operation and accurate result, and can be widely used for effect evaluations of duck hemorrhagic ovaritis inactivated vaccines.

Description

technical field [0001] The invention relates to a method for testing the efficacy of inactivated vaccines for veterinary use, in particular to a method for testing the efficacy of inactivated vaccines for duck hemorrhagic oophoritis, which belongs to the technical field of testing biological products for animals. Background technique [0002] Duck Hemorrhagic Ovaritis (DHO) is a newly popular, acute and highly contagious duck infectious disease. Classified as a member of the Flaviviridae, it infects ducks of different ages, causing ovarian hemorrhage, testicular atrophy, egg production, fertilization rate and hatching rate of sexually mature ducks, and increased duck death rate. [0003] In the study of the taxonomic status of the virus, through the sequence homology analysis and evolution analysis of the genome sequence of the F4 generation duck embryo passage virus of the isolated virus strain and known viruses, it was found that the virus is similar to the Tan The closes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15G01N33/569
Inventor 刘月焕杨保收韩春华何平有林健郁宏伟朱秀同刘浩毛雅园徐倩倩赵际成段会娟潘洁梁武
Owner INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI
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