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Method for obtaining leaf lettuce clustering analysis chart, primer composition and application

A technology of primer combination and cluster analysis, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/testing, etc., can solve the problems of effective identification of genetic diversity and kinship of lettuce germplasm resources that cannot be used for leaves , to achieve the effects of high polymorphism, wide sample adaptability and strong stability

Active Publication Date: 2014-04-30
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The embodiment of the present invention provides a method for obtaining the cluster analysis graph of lettuce for leaves, primer combination and application, which can solve the problem that the genetic diversity and genetic relationship of lettuce for leaves cannot be effectively identified at present

Method used

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  • Method for obtaining leaf lettuce clustering analysis chart, primer composition and application
  • Method for obtaining leaf lettuce clustering analysis chart, primer composition and application

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Experimental program
Comparison scheme
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Embodiment 1

[0021] The embodiment of the present invention provides a primer combination for obtaining a cluster analysis graph of lettuce for leaves, which is any one of the following primer combinations:

[0022] (1)E39 / M58

[0023] (2)E40 / M55

[0024] (3)E41 / M56

[0025] (4)E50 / M78

[0026] (5)E75 / M65

[0027] (7)E75 / M66

[0028] (8)E75 / M64

[0029] (9)E76 / M64

[0030] (10)E78 / M65

[0031] (11) E75 / M63.

Embodiment 2

[0033] The present embodiment also provides a method for obtaining a cluster analysis graph of leaf lettuce, the method comprising:

[0034] Using the genomic DNA of lettuce as a template, the primers of claim 1 are used for PCR amplification to obtain the amplified product;

[0035] Perform denatured polyacrylamide gel electrophoresis on the amplified product obtained in the above steps to separate DNA markers, and display DNA bands by silver staining;

[0036] Select the clearly identifiable electrophoretic bands on the electrophoresis plate, and use the unweighted group average method for cluster analysis to obtain the cluster analysis diagram.

[0037] The step of selecting the clearly identifiable electrophoretic bands on the electrophoretic plate and using the non-weighted group average method to perform cluster analysis to obtain the cluster analysis diagram in the above method can be specifically achieved in the following ways:

[0038] (1) Determine the band of genet...

Embodiment 3

[0067] Present embodiment is the explanation to the analysis and application of the cluster analysis diagram of leaf lettuce, specifically as follows:

[0068] According to the amplification results of 11 pairs of primers, the NTsys-pc2.10e software was used for processing, and the UpGMA method was used for cluster analysis.

[0069] When the similarity coefficient is 0.460, the 73 leaf lettuce test varieties are clustered into a large group. When the similarity coefficient is 0.590, the 73 materials can be clearly divided into two groups, and one group contains 4 materials. (denoted as A), another group contains 69 materials (denoted as B).

[0070] Group A was divided into three subgroups when the similarity coefficient was 0.832. It shows that they have a high degree of similarity within the group. Divide the two loose-leaf lettuces of S22 and W72 into a group, and the two ball lettuces of J40 and W63 are each divided into one group, and J40 is autumn day, which is a vari...

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Abstract

The present invention discloses a method for obtaining a leaf lettuce clustering analysis chart, a primer composition and an application, and belongs to the field of biological germplasm analysis. The primer combination for obtaining the leaf lettuce clustering analysis chart comprises an any one group selected from (1) E39 / M58, (2) E40 / M55, (3) E41 / M56, (4) E50 / M78, (5) E75 / M65 (7) E75 / M66, (8) E75 / M64, (9) E76 / M64, (10) E78 / M65, and (11) E75 / M63. Based on a molecular marker method AFLP in a PCR technology, the invention further discloses the leaf lettuce clustering analysis chart obtained by applying the special primer. The leaf lettuce clustering analysis chart obtained by using the method of the present invention has high polymorphism, and strong reliability and stability, and can provide important effects in identification of leaf lettuce genetic relationship, hybridization of different varieties, introductions between varieties in different areas, and other fields.

Description

technical field [0001] The invention relates to a method for obtaining a cluster analysis graph of leaf lettuce, primer combination and application. Background technique [0002] At present, my country's National Vegetable Germplasm Bank collects and preserves more than 200 leaf lettuce germplasm resources, but most of the cultivars used in production are imported from abroad. Leaf lettuce is a low-input, high-yield, fast-growing vegetable with rich nutritional value. In recent years, the cultivation area has expanded rapidly, and it has gradually become one of the first-choice green leafy vegetables for people to eat. However, the cultivated varieties of leaf lettuce in my country mainly rely on the introduction of existing foreign varieties, and the varieties and types with independent intellectual property rights are very limited, and there is almost a blank in the collection and identification of variety resources. Therefore, it is necessary to carry out the innovation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 范双喜韩莹琰刘甜甜李君平陈青君刘超杰
Owner BEIJING UNIV OF AGRI