Kit for chemilumineseent quantitative immunoassay of angiotensin I and preparation method thereof

A technique for chemiluminescence immunity and angiotensin, which is applied in the field of immunoassay medicine, can solve the problems of narrow detection range and low sensitivity, and achieve the effects of wide concentration range, high sensitivity and easy operation.

Active Publication Date: 2014-11-26
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The problem to be solved by the present invention is to provide a chemiluminescent immunoquantitative detection kit for angiotensin I and its preparation method, which solves the defects of low sensitivity and narrow detection range

Method used

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  • Kit for chemilumineseent quantitative immunoassay of angiotensin I and preparation method thereof
  • Kit for chemilumineseent quantitative immunoassay of angiotensin I and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Preparation of Angiotensin I Chemiluminescent Immunoquantitative Detection Kit

[0029] Angiotensin I chemiluminescent immunoquantitative detection kit, characterized in that the kit includes: AngI antibody-coated plate, horseradish peroxidase-labeled AngI, AngI calibrator, AngI quality control substance, luminescence solution A Solution and B solution, 20 times concentrated lotion.

[0030] Angiotensin Ⅰ Chemiluminescent Immunoquantitative Detection Kit was prepared by the following method

[0031] (1) Coating: Add AngI antibody to carbonate buffer (pH=9.6) and mix well, add 100uL to each well of a microplate, place in a refrigerator at 4°C overnight, wash with washing solution for five After the third time, the microwell plate was blocked with bovine serum albumin solution, and after discarding the liquid in the well, the microplate was dried and sealed to obtain a microwell plate coated with AngI antibody.

[0032] (2) Using the sodium periodate oxidatio...

Embodiment 2

[0057] Example 2: Preparation of Angiotensin I Chemiluminescent Immunoquantitative Detection Kit

[0058] The kit of the present invention was prepared in the same manner as in Example 1, except that the solid phase carrier was a 48-well microwell plate.

Embodiment 3

[0059] Embodiment 3: the using method of kit of the present invention

[0060] 1. Take the kit out of the 4°C refrigerator and equilibrate at room temperature (18-25°C) for 30 minutes.

[0061] 2. Prepare lotion: dilute the concentrated lotion with distilled water at 1:20 (1mL lotion plus 19mL distilled water). If the concentrated lotion has crystals, place the concentrated lotion at room temperature or 37°C until the crystals dissolve before diluting.

[0062] 3. Take out an appropriate amount of coated slats according to the needs of the experiment. Set 1 well for blank control, 2 wells for calibrator, and 10 wells for quality control. Add 50 μL of Ang I calibrator, quality control substance and sample to each well, and no calibrator, quality control substance and sample are added to the blank control.

[0063] 4. Add 50 μL of AngI enzyme conjugate to each well, except the blank control well.

[0064] 5. Gently oscillate by hand or machine for 10 seconds to mix well, cov...

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Abstract

The invention discloses a kit for chemilumineseent quantitative immunoassay of an angiotensin I. The kit comprises an Ang I antibody-coated plate, a horse radish peroxidase-labeled Ang I, an Ang I calibrator, Ang I quality control materials, a light-emitting solution A, a light-emitting solution B, and a washing liquid concentrated by 20 times. The invention also discloses a preparation method of the kit for chemilumineseent quantitative immunoassay of an angiotensin I. Compared with the existing kit, the kit provided by the invention can be operated more simply, is safe and does not produce pollution on the environment. In addition, the kit provided by the invention allows a wide range of tested sample concentrations and has a long reagent validity period and good stability.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine, in particular to an angiotensin I (AngI) chemiluminescence immunoquantitative detection kit and a preparation method thereof. Background technique [0002] Hypertension (Hypertension) is a common disease worldwide, with a prevalence as high as 10% to 20% around the world, and can lead to cerebrovascular, heart, and kidney lesions, and is a major disease that endangers human health. According to the latest data from the Ministry of Health, there are more than 200 million hypertensive patients in my country. With the development of the economy and the improvement of people's living standards, hypertension has increasingly become an important public health problem. [0003] Angiotensin Ⅰ (Angiotensin Ⅰ, Ang Ⅰ) is formed by the action of renin on angiotensinogen. Renin can catalyze the hydrolysis of the peptide bond between angiotensinogen leucine (Leu) and valine (Val) to produce the decapept...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
Inventor 刘萍张影宋启超范利花
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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