Diagnostic reagent kit for separating serum protein with ultra-thin agarose gel electrophoresis slab and preparation method of diagnostic reagent kit

Abstract

The invention discloses a diagnostic reagent kit for separating serum protein with an ultra-thin agarose gel electrophoresis slab, comprising the ultra-thin agarose gel electrophoresis slab which has the thickness smaller than 1mm, a sample loading film with equidistant micropores and a staining agent. According to the invention, a homemade plastic thin slab is used as a mold, the prepared agarose slab has the thickness smaller than 1mm which even reaches up to 0.8mm, is smooth in electrophoresis electrification, is shorted in staining time and is stained, has extremely short discoloration time and high protein separation resolution, and is coordinated with the sample loading film for spotting to replace the traditional sawtooth physical pore forming post spotting, and the operation is simple and convenient; and corresponding long strip-shaped micropores are prearranged according to the width of the slab, and the spotting effect is extremely good. Known from the experimental results of the application range of the reagent kit, the diagnostic reagent kit for separating the serum protein with the ultra-thin agarose gel electrophoresis slab can accurately detect albumin, alpha1-globulin, alpha2-globulin, delta-globulin and gamma-globulin. The invention provides an analysis product for a relevant serum protein electrophoresis separation spectrum in clinic practice, which is beneficial to the accurate diagnosis of diseases and has wide application prospect.

Description

technical field [0001] The invention relates to an ultra-thin agarose gel electrophoresis gel plate separation serum protein diagnostic kit and a preparation method thereof. Background technique [0002] The configuration method of the agarose gel electrophoresis plate in the prior art often has a thickness of more than 3mm, which not only affects the protein mobility, but also affects the staining effect, because the dye is not easy to penetrate if it is too thick, and the protein bands often form vacuoles, which is difficult for scanning. The quantitative results are directly affected; in addition, the decolorization time is also very long. In addition, for ultra-thin agarose electrophoresis gel plates (thickness < 1 mm), adding samples by leaving grooves on the gel plate through toothed sample loading molds will cause the electrophoresis gel plate to be damaged and cannot be used. As a result, the use of the agarose gel electrophoresis plate is not suitable for the di...

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Problems solved by technology

[0002] The configuration method of the agarose gel electrophoresis plate in the prior art often has a thickness of more than 3mm, which not only affects the protein mobility, but also affects the staining effect, because the dye is not easy to penetrate if it is too thick, and the protein bands often form vacuoles, which is difficult for scanning. Quantitative results are directly affected; in addition, the decolorization time is also very long

In addition, for ultra-thin agarose electrophoresis gel plates (thickness < 1mm), adding samples by leaving grooves on the gel plate through the toothed sample loading mold will cause the electrophoresis gel plate to be damaged and cannot be used

As a result, the use of agarose gel electrophoresis plates is not suitable for diagnostic kits for separating serum proteins, and the prices of existing products are relatively high

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