Detection method, probe set, and diagnostic kit for predicting postoperation recurrence-free survival of colorectal cancer via gene expression states of two probes

Abstract

The present invention discloses a detection method for predicting postoperation recurrence-free survival of colorectal cancers via gene expression states of two probes. The method includes: collecting cancerous tissue specimens excised in an operation of a colorectal cancer patient, extracting RNA samples, purifying the RNA samples, detecting the expressions of two specific probes, and calculating the number of a high expression probe to evaluate the patient's prognosis. The invention also discloses a probe set composed by the two probes, and a diagnostic kit containing the probe set. The detection method of the invention helps to improve the survival rate of the colorectal cancer patients. The risk degree of postoperative recurrence and metastasis occurrence of the patients can be quickly determined through the detection and the joint analysis of the two specific probes after that the colorectal cancer patients receive radical operations, so the detection method enables adjuvant treatments for the patients with a higher risk degree of recurrence to be done early to help prolong the survival time of the patients.

Description

Technical field [0001] The present invention relates to the prognosis field of colorectal cancer, in particular to a detection method for predicting recurrence-free survival after colorectal cancer operation based on the expression of two genes. The invention also relates to a probe set and a diagnostic kit used in the method. Background technique [0002] Colorectal cancer generally refers to malignant tumors that occur in the rectum and all colons (ascending colon, transverse colon, descending colon, sigmoid colon), and is one of the common malignant tumors in clinical practice. At present, there are about 800,000 new cases of colorectal cancer in China each year, and 500,000 people die from colorectal cancer each year. Due to changes in living conditions and habits, the incidence of colorectal cancer is still increasing year by year. Compared with the 1990s and 1970s, the incidence of colorectal cancer has increased by 31.95% in cities and 8.51 in rural areas. %. Among the f...

AI Technical Summary

Problems solved by technology

However, due to the lack of obvious early symptoms of colorectal cancer, the lack of specific clinical manifestations, and the poor self-care awareness of patients or the avoidance of medical treatment, early detection and diagnosis of colorectal cancer are relatively difficult. Clinically, more than 60-70% Most of the patients were diagnosed with advanced colorectal cancer, and among the stage II and stage III patients who can undergo radical surgery for colorectal cancer, about 20% and 50% of the patients will relapse and metastasize after operation, depending on the condition Adjuvant therapy such as chemotherapy or radiotherapy

However, tumor recurrence and metastasis involve many gene changes, and the process is quite complicated. Therefore, it is difficult to predict the risk of postoperative recurrence and metastasis of patients.

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