Method for degumming crude soybean oil by embed-crosslinked phospholipase A1 aggregate
A technology of cross-linked phospholipids and aggregates, applied in the direction of fat oil/fat refining, fat production, etc., can solve the problem of low activity, achieve high activity, easy operating conditions, and increase economic benefits
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specific Embodiment approach 1
[0006] Specific embodiment one: In this embodiment, embedding-cross-linked phospholipase A 1 Aggregate is used for the method for soybean crude oil degumming, is characterized in that using embedding-crosslinking phospholipase A 1 Aggregates degumming crude soybean oil through the following steps: 1. Embedding-cross-linking phospholipase A 1 Preparation of aggregates: Add 1 mL of enzyme to sodium dihydrogen phosphate and citric acid buffer solution with a pH value of 5.0, adjust the concentration of the enzyme solution to 0.02-0.07 g / mL, and slowly add the enzyme at 0°C to a saturation of 65-90 % ethanol, the precipitation reaction time is 30min under the condition of pH 3-8, and the stirring is continued to ensure that the particles of the enzyme aggregate are uniform, and then centrifuged at 10000r / min to collect the precipitate; cross-linked phospholipase A 1 The preparation and embedding process of aggregates: Add glutaraldehyde to the aggregate suspension at a concentrat...
specific Embodiment approach 2
[0007] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 2, a citric acid solution with a mass concentration of 40-45% and a mass of 1-1.8% of soybean crude oil is added to the processed soybean crude oil And stir at a speed of 600-1200r / min for 7-15min. Other steps are the same as in the first embodiment.
specific Embodiment approach 3
[0008] Specific embodiment three: the difference between this embodiment and specific embodiment one is that adding mass concentration to the processed soybean crude oil in step 2 is 45%, and the quality is a citric acid solution of 1.5% soybean crude oil and 1000r / Min speed stirring 12min. Other steps are the same as in the first embodiment.
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