Method for degumming crude soybean oil by embed-crosslinked phospholipase A1 aggregate

A technology of cross-linked phospholipids and aggregates, applied in the direction of fat oil/fat refining, fat production, etc., can solve the problem of low activity, achieve high activity, easy operating conditions, and increase economic benefits

Active Publication Date: 2015-01-28
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention is aimed at immobilized phospholipase A 1 low activity, and the proposed use of embedded-cross-linked enzyme aggregates to immobilize phospholipase A 1 , and the embedded-cross-linked phospholipase A 1 Aggregate is used for the method of soybean crude oil degumming

Method used

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specific Embodiment approach 1

[0006] Specific embodiment one: In this embodiment, embedding-cross-linked phospholipase A 1 Aggregate is used for the method for soybean crude oil degumming, is characterized in that using embedding-crosslinking phospholipase A 1 Aggregates degumming crude soybean oil through the following steps: 1. Embedding-cross-linking phospholipase A 1 Preparation of aggregates: Add 1 mL of enzyme to sodium dihydrogen phosphate and citric acid buffer solution with a pH value of 5.0, adjust the concentration of the enzyme solution to 0.02-0.07 g / mL, and slowly add the enzyme at 0°C to a saturation of 65-90 % ethanol, the precipitation reaction time is 30min under the condition of pH 3-8, and the stirring is continued to ensure that the particles of the enzyme aggregate are uniform, and then centrifuged at 10000r / min to collect the precipitate; cross-linked phospholipase A 1 The preparation and embedding process of aggregates: Add glutaraldehyde to the aggregate suspension at a concentrat...

specific Embodiment approach 2

[0007] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 2, a citric acid solution with a mass concentration of 40-45% and a mass of 1-1.8% of soybean crude oil is added to the processed soybean crude oil And stir at a speed of 600-1200r / min for 7-15min. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0008] Specific embodiment three: the difference between this embodiment and specific embodiment one is that adding mass concentration to the processed soybean crude oil in step 2 is 45%, and the quality is a citric acid solution of 1.5% soybean crude oil and 1000r / Min speed stirring 12min. Other steps are the same as in the first embodiment.

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Abstract

The invention relates to a method for degumming crude soybean oil by embed-crosslinked phospholipase A1 aggregate. In order to solve the problems that poor controllability of reaction conditions in practical production causes enzyme inactivation and enzyme inactivation is high in phospholipase cost, the embed-crosslinked phospholipase A1 aggregate is selected for degumming. The method includes the steps of firstly, preparing the embed-crosslinked phospholipase A1 aggregate; secondly, pre-treating the crude soybean oil; thirdly, adding the embed-crosslinked phospholipase A1 aggregate into the oil; and fourthly, centrifuging after mixing so as to finish degumming of the crude soybean oil. By the method for degumming the crude soybean oil compared with the existing methods, the degumming efficiency is increased by 5-25%, and economic benefit is increased by 1-3%.

Description

technical field [0001] The present invention relates to the use of embedded-cross-linked phospholipase A 1 Process for degumming crude soybean oil by aggregates. Background technique [0002] Cross-linked enzyme aggregates (CLEAs) are a new type of immobilized enzyme technology, which has the advantages of simple preparation, high recovery rate of enzyme activity, strong operation and storage stability, etc., and is widely used in the preparation of immobilized enzymes. In recent years, a series of new advances have been made in the combination of CLEAs technology with materials science, imprinting engineering, media engineering, and reaction engineering, including carrier-immobilized CLEAs, embedded CLEAs, imprinted CLEAs, multi-enzyme CLEAs, and membrane plasma reaction of CLEAs. Some achievements have been made in the fields of chiral molecule resolution and synthesis, antibiotic production, etc. The recovery rate of CLEAs of some enzymes is more than 100% relative to t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B3/00C11B3/04
Inventor 江连洲刘天一于殿宇张欢刘飞刘丹怡孙立斌解桂东关忠齐晓芬王立琦
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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