Method for the identification of animals vaccinated against brucella

A technology for brucellosis and brucellosis, applied in veterinary vaccines, vaccines, pharmaceutical formulations, etc., can solve problems such as failure to express

Inactive Publication Date: 2012-11-14
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC) +3
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  • Abstract
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  • Claims
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Problems solved by technology

However, they were unable to express it properly in rinderpest virus

Method used

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  • Method for the identification of animals vaccinated against brucella
  • Method for the identification of animals vaccinated against brucella
  • Method for the identification of animals vaccinated against brucella

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Experimental program
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Embodiment

[0060] Hereinafter, the present invention will be illustrated by measurements performed by the inventors.

[0061] 1- Development of a Fluorescent Vaccine Derivative Prototype (S19-GFPp) of Brucella abortus S19

[0062] The reference vaccine B. abortus S19 (obtained from OIE Brucellosis Reference Laboratory, AFSSA, France) was genetically modified by electroporation with a plasmid encoding GFP. The plasmid used was pBBR-2-gfp derived from plasmid pBBRIMCS-2, which contains a kanamycin-resistant insert (Kovach et al., 1995. Gene. Vol 166:175-176) and has Insert encoding the GFP gene under the control of the promoter. It is known that this plasmid constitutively produces GFP without integrating into the Brucella chromosome (Celli et al., 2003. Journal of Experimental Medicine, 198(4):545-556).

[0063] 2.- Genetic and microbiological characterization of S19-GFPp

[0064] 2.1. It has been found that the bacterial S19-GFPp emission can be obtained by directly illuminating the ...

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Abstract

The present invention relates to: the use of a strain of Brucella spp. expressing the green fluorescent protein (GFP) in the preparation of medicaments for the prevention of brucellosis in mammals, vaccines against brucellosis, and the method for identifying mammals to which said vaccines have been administered. Preferably, the method of the invention is an immunoassay, and, more preferably, it is an enzyme-linked immunosorbent assay (ELISA).

Description

technical field [0001] The present invention is in the field of preventive medicine and public health and relates to the development and use of genetically modified live vaccines against Brucella spp. Direct and indirect diagnostic techniques in animals. Background technique [0002] Brucellosis is a disease transmitted from animals to humans. In animals, Brucella infection causes abortion, sterility, reduced production and restrictions on the trade of animals and animal products, which is an animal health problem with economic implications. Furthermore, the bacterium is transmitted to humans from infected animals, causing a debilitating and often disabling disease, there is no vaccine against it, and its treatment for prolonged periods with frequent relapses requires high doses of antibiotics. [0003] Therefore, Brucella is an important public health problem. It has been shown that the prevalence of brucellosis in humans correlates directly with the prevalence of brucel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/10
CPCA61K39/098G01N2333/43595A61K2039/545A61K2039/552G01N2333/23C07K14/43595
Inventor 玛丽亚·格里洛多尔赛特比阿特丽斯·阿莫雷纳扎巴尔扎达米安·德安德烈斯卡拉埃德加·莫雷诺罗布莱斯埃斯特班·查维斯奥拉特卡泰丽娜·古兹曼韦里卡洛斯·洽孔迪亚兹
Owner CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
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