Esophageal cancer marker

A technology of esophageal cancer and composition, applied in the field of esophageal cancer markers, can solve the problems of not finding esophageal cancer-specific, early-stage esophageal cancer, and difficult-to-carcinoma-specific transporters

Inactive Publication Date: 2012-11-28
BEIJING B&M BIOTECH CO LTD +1
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  • Summary
  • Abstract
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Problems solved by technology

However, transporters reported so far, including LAT1, are expressed in small amounts in normal cells, and it is difficult to say that they are cancer-specific transporters (Non-Patent Documents 3 and 4)
[0007] As mentioned above, so far, no markers that are highly specific for esophageal cancer or have a high positive rate for early-stage esophageal cancer have been found.

Method used

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  • Esophageal cancer marker
  • Esophageal cancer marker
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preparation example Construction

[0110] The preparation of polyclonal antibodies can be carried out according to the following steps. First, an antigen (for example, the full length or a part of the SLC38A4 protein described in SEQ ID NO: 1) is prepared, and then animals such as rabbits are immunized with the antigen. The SLC38A4 protein as an antigen can be prepared by separating and purifying it from a biological sample. In addition, SLC38A4 protein prepared as a recombinant protein can also be used as an antigen. The recombinant protein is prepared by inserting the gene encoding the SLC38A4 protein or a part thereof into a vector in an expressible state, introducing the vector into an appropriate host, and then isolating and purifying the protein expressed in the host. The SLC38A4 protein, preferably the peptide region comprising the 29th to 47th amino acid sequence "GIGNSEKAAMSSQFANEDT" (SEQ ID NO. 2) can be prepared to combine with GST, β-galactosidase, maltose binding protein, histidine ( A fusion pro...

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Abstract

As a result of immunohistochemical staining of human cancer pathological tissue sections and normal tissue sections with various marker candidates, the antibody against the SLC38A4 protein, out of 80 spontaneously developed types of antigen-specific antibodies against a transporter, was found to react specifically to esophageal cancer tissue and in particular to show high reactivity in esophageal cancer tissue in early well-differentiated stages.

Description

technical field [0001] The present invention relates to a method for examining esophageal cancer using expression of SLC38A4 (alias: SANT4) protein as an index, a composition for detecting esophageal cancer comprising an anti-SLC38A4 protein antibody, and a method for producing the composition for detecting esophagus comprising anti-SLC38A4 A protein antibody detection kit for esophageal cancer, and a method for screening a drug for treating esophageal cancer using the SLC38A4 protein. Background technique [0002] Esophageal cancer is a tumor of epithelial origin that occurs in the esophagus. Histologically, it is mainly classified into squamous cell carcinoma of esophageal mucosal epithelium and adenocarcinoma of esophageal adenocarcinoma. In Japan, squamous cell carcinoma accounts for 90% of all esophageal cancers, and the remaining 5% is adenocarcinoma. It can be seen from this that Japanese esophageal cancer is basically squamous cell carcinoma. Moreover, it mostly o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574C07K16/32G01N33/15G01N33/50
CPCG01N2333/47G01N33/5088G01N33/57407C07K16/30
Inventor O・吾守尔岸义朗
Owner BEIJING B&M BIOTECH CO LTD
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