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Identifying Viral Cell Tropism

一种细胞、病毒的技术，应用在HIV病毒领域，能够解决不能提供HIV-2型病毒特征测试等问题

Inactive Publication Date: 2012-12-05
CENT NAT DE LA RECH SCI (C N R S)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, a profile test for HIV-2 virus cannot be provided

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  • Identifying Viral Cell Tropism
  • Identifying Viral Cell Tropism
  • Identifying Viral Cell Tropism

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Embodiment Construction

[0018] The term "miRNA" or "microRNA" refers to a class of RNAs, usually 20 to 25 nucleotides in length, involved in the transcription of certain genes by degrading or blocking the translation of mRNAs derived from those transcriptions post adjustment. A "target mRNA" of a miRNA refers to an mRNA known or determined to be degraded or translationally blocked by said miRNA. miRNA in Griffiths-Jones ((2004) Nucleic Acids Res.32:D109-D111), Griffiths-Jones et al. ((2008) Nucleic Acids Res 36:D154-D158) and miRA database (miRBase, http: / / microRNA. specifically described in sanger.ac.uk).

[0019] The expression "virus" as used herein includes all types of viruses. In particular, the virus may be selected from the group of more or less pathogenic viruses of their variants or varieties, such as retroviruses, especially HIV viruses, influenza viruses, coronaviruses, measles viruses, herpes viruses (including EBV , herpes simplex virus and CMV virus), papillomavirus. Preferably, th...

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Abstract

The invention relates to an in vitro method for identifying microRNA or the target mRNAs thereof, the expression of which, during the infection of cells by a virus using a cell receptor and at least one cell co-receptor in order to enter the cell, is specifically modified on the basis of the cell co-receptor used by the virus for the entry thereof into the cells, said method including: i) determining the microRNA expression levels in a test cell, expressing a receptor, a first co-receptor, and at least one other co-receptor, after infection by a first virus using the first co-receptor and by at least one other virus using another co-receptor, respectively; ii) identifying the microRNAs, the expression level of which is modulated during the infection by each of the viruses in relation to the expression level in the uninfected cells; iii) comparing the thus-identified mircoRNAs ; iv) selecting the microRNAs, the modification of the expression level of which is specific to the use of a co-receptor; and v) optionally identifying the target mRNAs of the thus-selected microRNAs.

Description

technical field [0001] The present invention relates to methods for characterizing the cellular tropism of viruses, especially human immunodeficiency virus (HIV), especially HIV viruses that utilize the CXCR4 and CCR5 receptors for cell entry. Background technique [0002] HIV virus entry into cells involves several viral proteins, including the envelope proteins gp41 and gp120. The first step in the HIV viral replication cycle involves viral binding to helper T4 lymphocytes through the interaction of the gp120 protein with CD4 cell proteins. In addition, in order for the fusion of the virus and the cell membrane to occur, the HIV virus needs to interact with a cellular co-receptor. The most important co-receptors in vivo are the receptors for the chemokines CXCR4 and CCR5. The utilization of different co-receptors is associated with time-dependent changes in immunodeficiency and thus infection: at the onset of infection, the so-called R5 virus interacts with the CCR5 co-r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6813
Inventor 查尔斯-亨利·勒塞利耶瓦莱丽·库尔尼奥曼努埃拉·布捷迪亚娜·德康吉勒·科兰
Owner CENT NAT DE LA RECH SCI (C N R S)
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