Preservation culture medium for hypsizygus marmoreus stock culture and method for preparing preservation culture medium

Abstract

The invention relates to the field of edible fungi culture, and in particular relates to a preservation culture medium for a hypsizygus marmoreus stock culture and a method for preparing the preservation culture medium. Firstly, the preservation culture medium for the hypsizygus marmoreus stock culture is provided, and has a culture medium formula by weight percent as follows: 50-55% of wood flour, 30%-40% of bran, 0.5%-1% of lime powder and 5%-10% of agar. The method for preparing the preservation culture medium is that the wood flour and the bran are mixed according to the proportion to be ground into particles, and then, the lime powder is added to mix uniformly; and the agar is heated to melt and then poured into the mixture of the wood flour, the bran and the lime powder, and water is appropriately added to make the water content of the mixture to be 55-60%. The hypsizygus marmoreus stock culture medium which is prepared by the method is simple and convenient to prepare and low in cost, and has longer preservation time; and moreover, the fungi activity is more strongly kept, the inoculated fungi has quick spawn running, disease resistance and higher heterozygosity resistance, and higher fungi yield of a single bottle is achieved.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to a culture medium for preserving mother species of shimeji mushroom and a preparation method thereof. Background technique [0002] At present, when people preserve the mother species of jiji mushroom, they generally use PADA medium and store them at a temperature of 4-8°C. But its production is loaded down with trivial details, and storage time is shorter, and the aging and degeneration of strain easily takes place, causes the loss that is difficult to predict to production, seriously restricts the production of shimeji mushroom and the benefit of enterprise. Contents of the invention [0003] The object of the present invention is to provide a preservation medium for mother species of shimeji mushrooms which is easy to make, has a long storage time and is suitable for keeping the activity of shiji mushrooms, and a preparation method thereof. [0004] Firstly, a kind ...

AI Technical Summary

Problems solved by technology

But its production is loaded down with trivial details, and storage time is shorter, the aging and degeneration of bacterial strain easily occur, cause the loss that is unpredictable to production, seriously restrict the production of true jiji mushroom and the benefit of enterprise