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Bacillus sphaericus parasporal crystal protein preparation method and application thereof

A technology of parasporal crystal protein and Bacillus sphaericus, which is applied to microbial metabolites and their application fields, can solve problems such as enhancing the toxicity of BinA protein, and achieve the effects of improving activity, increasing yield and high efficacy

Inactive Publication Date: 2014-01-29
XI AN JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

BinB alone is non-toxic to mosquito larvae, but its presence significantly enhances the toxicity of BinA protein. Only when the two proteins exist at the same time can the toxin protein exert its maximum killing activity. It is a binary toxin (Binary toxin, Bin)

Method used

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  • Bacillus sphaericus parasporal crystal protein preparation method and application thereof
  • Bacillus sphaericus parasporal crystal protein preparation method and application thereof
  • Bacillus sphaericus parasporal crystal protein preparation method and application thereof

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Embodiment Construction

[0036] The method for preparing Bacillus sphaericus parasporal crystal protein and its application provided by the present invention, through batch fermentation of wild-type Bacillus sphaericus, the cells are lysed and centrifuged to obtain its metabolite parasporal crystal protein. The protein is applied to anti-human tumor cells, and the result shows that it can be applied to the preparation of anti-tumor drugs. The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0037] 1. Bacillus sphaericus IAB 872 strain:

[0038] Bacillus sphaericus IAB 872 is an existing Bacillus sphaericus that produces a toxin that is commonly used to kill mosquitoes. Specifically, the present invention is specifically described by a strain of Bacillus sphaericus IAB 872 isolated from a soil sample. Of course, those skilled in the art can also use other channels to obtain B...

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Abstract

The invention discloses a Bacillus sphaericus parasporal crystal protein preparation method and application thereof. Wild type Bacillus sphaericus IAB 872 is cultured, and ultrasonic treatment, lysozyme treatment, DTT (dithiothreitol) treatment and compound cardiografin treatment are performed to improve the yield and activity of soluble crystal proteins, thereby obtaining high-effect soluble crystal proteins as many as possible. The Bacillus sphaericus parasporal crystal proteins have a certain inhibiting effect on human malignant tumor cells, and can be used for the preparation of antitumor drugs. The inhibition of the Bacillus sphaericus parasporal crystal proteins on the proliferation of human tumor cells includes the inhibition on tumor cells, the influence on apoptosis promotion of tumor cells, the influence on G2 / M phase of cells and the arrest of cells in G2 / M phase.

Description

technical field [0001] The invention belongs to the technical field of microbial metabolites and applications thereof, and relates to a method for preparing Bacillus sphaericus parasporal crystal protein and its application. Background technique [0002] Bacillus sphaericus is a strictly aerobic bacillus. The growth cycle can be divided into vegetative phase and spore phase. The spores are born at or near the end of the cell. During the process of spore formation, one end of the thallus expands, and the sporangia Drumstick-shaped. In the cells of Bacillus sphaericus, with the formation of spores, some strains can produce one or more paraspore crystals, while others do not produce paraspore crystals, and the shape, size and number of paraspore crystals also vary with the strain. different from each other. Paraspore crystals and spores are attached to the same end of the cell. After the sporangia matures, the crystals and spores are still combined and wrapped in the outer me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C07K14/32C07K1/14A61K38/16A61P35/00C12R1/07
Inventor 罗文娟韩蓓张瑞娟刘翠翠张杰王嗣岑展颖转侯晓芳
Owner XI AN JIAOTONG UNIV
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