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Use of hla-g subtypes as markers of osteogenesis

A technology of HLA-G and HLA-G5, applied in the field of new osteogenic markers, can solve the problem of unsatisfactory osteogenic markers

Inactive Publication Date: 2016-04-27
COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] Thus, currently used osteogenic markers are not fully satisfactory

Method used

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  • Use of hla-g subtypes as markers of osteogenesis
  • Use of hla-g subtypes as markers of osteogenesis
  • Use of hla-g subtypes as markers of osteogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1: Confirmation of HLA-G Expressed by Osteoblasts

[0090] 1) Materials and methods

[0091] Cells and Cell Culture :

[0092] Adult bone marrow (BM) (MO in French) samples were collected from healthy volunteers undergoing plastic surgery. The samples were obtained on the recommendation of the ethics committee of the Trousseau CHU [University Teaching Hospital Center] in Tours, France. Mononuclear cells (MNC (CMN in French)) were inoculated in a culture medium supplemented with 10% fetal calf serum (FCS) (SVF in French) (PerbioHyclone; Logan) and 1% penicillin / streptomycin (InVitrogen Ltd; Paisley, UK) α-MEM medium (MEM). On day 14, at 80% confluence, mesenchymal stem cells (MSCs) were split in two and expanded. All experiments were performed with at least 3 different donors.

[0093] The SaOs2 osteosarcoma cell line (ECECC, Salisbury, UK) was cultured under the same conditions as for MSC expansion.

[0094] Induction of Osteoblasts and Chondroblas...

Embodiment 2

[0136] Example 2: Confirmation that osteoblasts from osteosarcoma express HLA-G

[0137] 1) Materials and methods

[0138] cell :

[0139] Osteosarcoma cell lines: HOS-154732 (McAllister et al., 1971), U2OS (Ponten et al., 1967), MG-63 (Billiau et al., 1977), SaOS2 (Fogh et al., 1975), CAL72 (Rochet et al. , 1999) and SaOS2RUNX2DNN (Ghali et al., 2010).

[0140] The various biopsies studied were obtained from the Department of Pathoanatomy at TrousseauCentreHospitalierUniversitaire (CHU) [teaching center of the University Hospital of Tours (France)].

[0141] Mesenchymal stem cells (MSC) (CSM in French):

[0142] The MSCs used were from healthy donors treated at the Orthopedic Traumatology Department of the CHU in Tours (France) and admitted for implantation in total hip replacement. Written informed consent was obtained from the patient. During the procedure 20 ml of bone marrow was taken from behind the iliac crest. These cells were used as controls for all tes...

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Abstract

The present invention relates to the use of at least one HLA-G subtype as a marker for assessing osteogenesis in mammals.

Description

technical field [0001] The present invention relates to a new marker of osteogenesis and its use in a method for evaluating osteogenesis in mammals. Background technique [0002] Osteogenesis is a process of bone tissue formation and development. Bones are formed from: [0003] - extracellular matrix of bone (approximately 22% to 25%), which is an organic matrix consisting essentially of type I collagen, but also containing other proteins such as osteonectin and osteocalcin, [0004] - a mineral matrix very rich in calcium (approximately 70%), [0005] - Two types of bone cells: osteoblasts (osteoblasts, bone cells, and lining cells derived from mesenchymal stem cells) and osteoclasts (cells derived from hematopoietic stem cells), and [0006] - Water (5% to 8%). [0007] Osteoblasts are cuboidal or cylindrical mononuclear epithelioid cells that are present in growing bone tissue. It characteristically expresses collagen type I, alkaline phosphatase (ALP (PA in French) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/74
CPCG01N33/68G01N33/74G01N33/6893
Inventor F·德沙索L·桑塞贝N·鲁阿-弗雷斯A·纳吉E·D·卡罗塞拉
Owner COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
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