Double-bioenzyme modified blending biological material containing gelatin and chitosan and preparation method and application thereof
A biomaterial, chitosan technology, applied in medical science, absorbent pads, prostheses, etc., can solve problems affecting the normal tissue growth of receptors, improve stability and mechanical properties, reduce production costs, stabilize performance and The effect of improving mechanical properties
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[0030] Example 1:
[0031] A method for preparing a blended biological material using dual biological enzymes to modify gelatin and chitosan, which comprises the following specific steps:
[0032] 1) Preparation of solution:
[0033] Weigh 2g of gelatin and add it to 50mL of deionized water, stir in a water bath at 50°C for 1 hour until the gelatin is completely dissolved to obtain a gelatin solution with a concentration of 0.04g / mL; weigh 1g of chitosan and add it to 50mL of 1% (volume percent v) / v%) acetic acid solution, stir at room temperature for 3 hours until the chitosan is fully dissolved to obtain a chitosan solution with a concentration of 0.02g / mL, and slowly add 1mol / L sodium hydroxide solution to the chitosan solution dropwise until The pH of the solution was 5.5; the microbial transglutaminase was added to deionized water and stirred at room temperature for 10 minutes, and then filtered to obtain a 2U / mL microbial transglutaminase solution; tyrosinase was added...
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[0046] Example 2:
[0047] A method for preparing a blended biological material using dual biological enzymes to modify gelatin and chitosan, which comprises the following specific steps:
[0048] 1) Preparation of solution:
[0049] Weigh 3 g of gelatin and add it to 50 mL of deionized water, stir in a water bath at 40°C for 1.5 hours until the gelatin is completely dissolved to obtain a gelatin solution with a concentration of 0.06 g / mL; weigh 1.5 g of chitosan and add it to 50 mL of 1% ((volume) Percent v / v%) acetic acid solution, stir at room temperature for 4 hours until chitosan is fully dissolved to obtain a chitosan solution with a concentration of 0.03g / mL, and slowly add 0.5mol / L hydroxide to the chitosan solution dropwise Sodium solution until the pH of the solution is 5.5; add microbial transglutaminase to deionized water and stir at a temperature for 15 minutes, and then filter to obtain 2U / mL microbial transglutaminase solution; add tyrosinase to PBS (pH 7 .0) ...
Example Embodiment
[0054] Example 3:
[0055] A method for preparing a blended biological material using dual biological enzymes to modify gelatin and chitosan, which comprises the following specific steps:
[0056] 1) Preparation of solution:
[0057] Weigh 6g of gelatin and add it to 100mL of deionized water, stir in a water bath at 60°C for 1.5 hours until the gelatin is completely dissolved to obtain a gelatin solution with a concentration of 0.06g / mL; weigh 2g of chitosan and add it to 100mL of 1% ((volume percent) v / v%) acetic acid solution, stir at room temperature for 5 hours until the chitosan is fully dissolved to obtain a chitosan solution with a concentration of 0.02g / mL, and slowly add 0.8mol / L sodium hydroxide dropwise to the chitosan solution The pH of the solution was 5.5; the microbial transglutaminase was added to deionized water and stirred at room temperature for 20 minutes, and then filtered to obtain a 2U / mL microbial transglutaminase solution; tyrosinase was added to PBS ...
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