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Method for extracting coix seed protein by enzymolysis

An extraction method and technology of barley, applied in the field of enzymatic hydrolysis and extraction of barley protein, can solve the problems of low yield, peculiar smell and low purity of barley protein, and achieve the effects of good product purity, short reaction time and high reaction efficiency

Inactive Publication Date: 2012-12-26
福建希昂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method consumes a large amount of acid and alkali solution, and the yield of barley protein is low, the purity is low and there is peculiar smell

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Crush 120 kg of barley to below 80-120 mesh, mix with 0.01 kg of EM compound enzyme, adjust the pH value to 5-7, control the ultrasonic frequency at 15KHz, react at 39°C for 6.5 hours, filter, and take the precipitate ;Put the precipitate in a far-infrared constant temperature drying oven, dry it for 2.5 hours at a constant temperature of 45°C, and finally get 11.8 kg of barley protein; if the protein content in barley is calculated according to the lowest value, it is 16%, so this experiment The yield is 61.46%.

Embodiment 2

[0030] Crush 120 kg of barley to below 80-120 mesh, mix with 0.0012 kg of EM compound enzyme, adjust the pH value to 5-7, control the ultrasonic frequency at 55KHz, react at 25°C for 4 hours, filter, and take the precipitate ; Place the precipitate in a constant-temperature far-infrared drying oven, dry it for 2 hours at a constant temperature of 40°C, and finally obtain 11.2 kg of barley protein, with a yield of 58.33%.

Embodiment 3

[0032] Crush 120 kg of barley to below 80~120), mix with 0.12 kg of EM compound enzyme, adjust the pH value to 5~7, control the ultrasonic frequency at 120KHz, react at 55°C for 10 hours, filter, and take the precipitate Place the precipitate in a constant-temperature far-infrared drying oven, dry it for 3 hours at a constant temperature of 50°C, and finally obtain 13.1 kg of barley protein, with a yield of 68.23%.

[0033] From the above, it can be seen that the yield of extracting barley protein by the method of the present invention is obviously higher than that of the traditional acid-base method.

[0034] The present invention uses conditions such as ultrasonic waves, temperature control, and pH value adjustment to assist the enzymolysis of barley, so that the reaction time of the enzymolysis process is short and the reaction efficiency is high; in addition, the enzymolysis is carried out by using EM compound enzymes, and the EM compound enzymes have the characteristics of...

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PUM

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Abstract

The invention provides a method for extracting coix seed protein by enzymolysis, which comprises the following steps: pulverizing coix seed, and mixing the pulverized coix seed with an EM composite enzyme water solution; under the conditions of ultrasonic treatment and heating, regulating the pH value of the mixed solution, and fermenting to finally form a fermentation mixed solution; filtering the fermentation mixed solution, and taking the precipitate; and putting the precipitate in an far-infrared constant-temperature drying box, and drying at constant temperature to finally obtain the coix seed protein. The invention has the advantages of simple and controllable reaction process, high reaction yield and high product purity.

Description

【Technical field】 [0001] The invention relates to an enzymatic extraction method of barley protein. 【Background technique】 [0002] Barley, also known as coix seed, coix seed rice, medicinal corn, etc., is the mature seed kernel of Coix lachry-majobi L., one of the oldest crops in my country. Barley is planted in most parts of our country, mainly in Fujian, Liaoning, Hebei, Guangdong, Hainan and other places. Barley not only has comprehensive nutritional components, including 16% to 21% protein, 4% to 6% fat, and 79.2% carbohydrates, but also contains 18 kinds of amino acids needed by the human body, multiple vitamins and minerals, and has very high nutritional value. It ranks first among undergraduate plants and has the reputation of "the king of grasses in the world". At present, the research and development of coix seed resources at home and abroad are very important, but the research is mainly concentrated on the extraction process and biological function of coixene es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/14
Inventor 黄建康
Owner 福建希昂医药科技有限公司