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Biological mineralization method of tussah silk fibroin for promoting cell growth

A technology of biomineralization and tussah silk is applied in the processing field of tussah silk fibroin to achieve the effects of short production cycle, no cytotoxicity and simple operation

Active Publication Date: 2014-01-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To sum up, there is no biomineralization method that is simple in process, easy to operate, and uses bionic methods to prepare tussah silk fibroin with high biocompatibility.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The biomineralization method of tussah silk fibroin that promotes cell growth in this embodiment includes the following steps in sequence.

[0030] (1) Weigh 10g of tussah silk fibroin cocoons, cut them into pieces, and put them into 1L boiling Na 2 CO 3 degumming treatment in aqueous solution, in Na 2 CO 3 In aqueous solution, Na 2 CO 3 The mass percentage is 0.5%, degumming treatment 30min, repeat above-mentioned operation once, clean with deionized water, dry and obtain 7.3g tussah silk fibroin fiber again.

[0031] (2) Take 5g of tussah fibroin fiber in step (1), add it to 35ml of LiSCN solution with a concentration of 9mol / L and dissolve evenly to obtain solution A, which is a mixed solution of tussah silk fibroin and LiSCN. Then solution A was dialyzed in deionized water for 3 days to obtain solution B, which was freeze-dried to make tussah silk powder, and 0.5g tussah silk powder was added to 9.5g hexafluoroacetone solution to dissolve tussah silk soluti...

Embodiment 2

[0038] The biomineralization method of tussah silk fibroin that promotes cell growth in this embodiment includes the following steps in sequence.

[0039] (1) Weigh 10g of tussah silk fibroin cocoons, cut them into pieces, and put them into 1L of boiling Na 2 CO 3 degumming treatment in aqueous solution, in Na 2 CO 3 In aqueous solution, Na 2 CO 3 The mass percentage is 0.5%, degumming treatment 30min, repeat above-mentioned operation once, clean with deionized water, dry and obtain 7.3g tussah silk fibroin fiber again.

[0040] (2) Take 5g of tussah fibroin fiber in step (1), add it to 35ml of LiSCN solution with a concentration of 9mol / L and dissolve evenly to obtain solution A, which is a mixed solution of tussah silk fibroin and LiSCN. Then solution A was dialyzed in deionized water for 3 days to obtain solution B, solution B was freeze-dried to make tussah fibroin powder, 1g tussah silk powder was added to 9g hexafluoroacetone solution, and tussah silk solution wa...

Embodiment 3

[0047] The biomineralization method of tussah silk fibroin that promotes cell growth in this embodiment includes the following steps in sequence.

[0048] (1) Cut the cocoons of tussah silkworms into pieces, and add Na with a mass percentage of 0.4% at a bath ratio of 1:25 2 CO 3 The degumming treatment is carried out in the aqueous solution, and after the degumming treatment for 25 minutes, it is cleaned with deionized water, and then dried to obtain the tussah silk fibroin fiber.

[0049] (2) Add the tussah silk fiber in step (1) into LiSCN aqueous solution with a concentration of 9.6mol / L and dissolve it evenly to obtain solution A. Put solution A into the dialysis membrane, and then dialyze in deionized water for 2 days Obtain solution B, then freeze and dry the solution B to make tussah silk fibroin powder, add tussah silk fibroin powder with 1 part by weight into hexafluoroacetone with 11 parts by weight for dissolution to obtain tussah silk fibroin powder Silk solutio...

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Abstract

The invention relates to a biological mineralization method of tussah silk fibroin for promoting cell growth. No existing biological mineralization method capable of preparing the tussah silk fibroin with high biocompatibility is easy to operate and quick. The method sequentially comprises the following steps: pulverizing tussah cocoon, and adding into a Na2CO3 water solution for degumming, thereby obtaining the tussah silk fibroin fiber; adding the tussah silk fibroin fiber into an LiSCN water solution, dissolving, dialyzing, freezing, drying to obtain tussah silk fibroin powder, and dissolving the tussah silk fibroin powder in hexafluoroacetone to obtain a tussah silk fibroin solution; dropwisely adding the tussah silk fibroin solution onto a plastic board, and drying in air to obtain a tussah silk fibroin film; and impregnating the tussah silk fibroin film in a CaCl2 water solution, cleaning with water and acetone, drying in air, and impregnating in an Na2HPO4 water solution, cleaning with water and acetone, drying in air, and repeating the operation 20-35 times, thereby obtaining the finished product. The invention has the advantages of simple technique and short preparation period; and the prepared biological material does not have cytotoxicity.

Description

technical field [0001] The invention relates to a biomineralization method of tussah silk fibroin, in particular to a biomineralization method of tussah silk fibroin that promotes cell growth, and belongs to a processing method of tussah silk fibroin protein. Background technique [0002] Tussah silkworm is a kind of wild silkworm, and my country's annual tussah silkworm production accounts for more than 90% of the world's total. Tussah silk fibroin is a natural polymer protein. Many studies have shown that tussah silk fibroin has good biocompatibility and mechanical strength. It is a degradable biopolymer material. Its degradation products have no toxic side effects on cells or the body. There is also little immune rejection or inflammatory response. Compared with silkworm silk fibroin, tussah silk fibroin is different in amino acid composition. Tussah silk fibroin protein is rich in alanine, glycine and serine, and the content of these three amino acids accounts for 80% o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/00C08J5/18C08L89/00C08K3/32
Inventor 杨明英帅亚俊朱良均闵思佳
Owner ZHEJIANG UNIV
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