Preparation method and application of composite probiotics fermentation Chinese medicinal herb composite feed additive
A technology of feed additives and fermented Chinese herbal medicines, applied in applications, animal feeds, animal feeds, etc., can solve problems such as low immunity and disease resistance, decreased feed digestion and absorption, and prone to large-scale diarrhea. Injury to intestinal mucosa, maintaining intestinal microecological balance, and maintaining optimal immune status
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Embodiment 1
[0024] Embodiment 1, a kind of production method of compound probiotic fermentation Chinese herbal medicine feed additive comprises the following steps:
[0025] (1) Preparation of compound Chinese herbal medicine: Weigh the Chinese herbal medicine according to the formula (Pulsatilla 15-25%, Myrobalan 25-35%, Glycyrrhizae 10-15%, Schisandra 25-35%), and crush them to 40-60 mesh , and mix evenly to prepare the compound Chinese herbal medicine.
[0026] (2) Liquid fermentation: take a 50L fermentation tank as a platform, and the liquid content is 35-40%, with (soluble starch 3.0%, bean cake powder 2.0%, potassium dihydrogen phosphate 0.3%, MgSO4.7H2O0.02%, CaCl20. 01%, the pH is adjusted to 7.0~7.2) as the medium, and the prepared compound Chinese herbal medicine powder is added at a ratio of 8-10%, at a temperature of 28-30°C and a speed of 300r.min -1 , Bacillus cereus (China Agricultural Microorganism Culture Collection Management Center, strain number: ACCC11109) inoculum ...
Embodiment 2
[0028] Embodiment 2, a kind of production method of compound probiotic fermentation Chinese herbal medicine feed additive, comprises the following steps:
[0029] (1) Preparation of compound Chinese herbal medicine: Weigh the Chinese herbal medicine according to the formula (Pulsatilla 15-25%, Myrobalan 25-35%, Licorice 10-15%, Schisandra 25-35%), crush it to 50 mesh, mix well , to prepare compound Chinese herbal medicine.
[0030] (2) Liquid fermentation: take the 50L fermenter as the platform, the liquid content is 40%, with (soluble starch 3.0%, bean cake powder 2.0%, potassium dihydrogen phosphate 0.3%, MgSO4.7H2O0.02%, CaCl20.01% , pH adjusted to 7.0-7.2) as the culture medium, adding the prepared compound Chinese herbal medicine powder at a ratio of 15%, at a temperature of 32°C and a speed of 200-250r.min -1, Bacillus cereus (China Agricultural Microorganism Culture Collection Management Center, strain number: ACCC11109) inoculum amount 3%, Bacillus subtilis (China Agr...
Embodiment 3
[0032] Embodiment 3, a kind of production method of compound probiotic fermentation Chinese herbal medicine feed additive, comprises the following steps:
[0033] (1) Preparation of compound Chinese herbal medicine: Weigh the Chinese herbal medicine according to the formula (Pulsatilla 15-25%, Myrobalan 25-35%, Licorice 10-15%, Schisandra 25-35%), crush it to 60 mesh, mix well , to prepare compound Chinese herbal medicine.
[0034] (2) Liquid fermentation: take a 50L fermentation tank as a platform, and the liquid content is 50-60%, with (soluble starch 3.0%, bean cake powder 2.0%, potassium dihydrogen phosphate 0.3%, MgSO4.7H2O0.02%, CaCl20. 01%, the pH is adjusted to 7.0~7.2) as the culture medium, and the prepared compound Chinese herbal medicine powder is added at a ratio of 20%, at a temperature of 35-37°C and a speed of 150-200r.min -1 , Bacillus cereus (China Agricultural Microorganism Culture Collection Management Center, strain number: ACCC11109) inoculum amount 3%, ...
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