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Method for measuring pollen vitality of lycoris plants using in-vitro germination method

A technique for in vitro germination of Lycoris plants, applied to biochemical equipment and methods, and microbial measurement/inspection, which can solve problems such as the method of not finding the pollen vitality of Lycoris genus

Inactive Publication Date: 2013-01-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the studies on Lycoris pollen at home and abroad focus on the systematic relationship and interspecies classification of Lycoris, and no effective method for identifying the pollen vigor of Lycoris has been found.

Method used

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  • Method for measuring pollen vitality of lycoris plants using in-vitro germination method
  • Method for measuring pollen vitality of lycoris plants using in-vitro germination method
  • Method for measuring pollen vitality of lycoris plants using in-vitro germination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Put the uncracked anthers of Chrysanthemum japonica into a dry petri dish filled with sulfuric acid paper, and place the petri dish in an indoor desiccator filled with color-changing silica gel for 4 days until the pollen disperses.

[0034] (2) Use a dissecting needle to take an appropriate amount of pollen into a concave glass slide, gently add 2-3 drops of culture solution containing 50 g / L sucrose and 20 mg / L boric acid, and mix gently.

[0035] (3) Gently put the slide glass into a humid box with a relative humidity of 80%, and place it in an environment of 27° C. for 60 min.

[0036] (4) Gently take out the glass slide to prevent violent vibration and prevent the pollen tube from breaking, and place it under a 60-fold microscope to observe the pollen germination. Vigorous pollen can be observed in the field of vision to germinate pollen tubes, which are about the length of the pollen grain itself 1 to 4 times, at this time it is convenient to count the pollen ...

Embodiment 2

[0045] (1) Evenly put the Hudixiao anthers that are about to crack into a dry petri dish filled with sulfuric acid paper, and place the petri dish in an indoor desiccator for 4 days until the pollen disperses.

[0046] (2) Use a dissecting needle to take an appropriate amount of pollen into the concave glass slide, gently add 2-3 drops of culture solution containing 40 g / L sucrose and 20 mg / L boric acid, and mix gently.

[0047] (3) Gently put the slide glass into a humid box with a relative humidity of 80%, and place it in an environment of 30° C. for 40 minutes.

[0048] (4) Gently take out the glass slide to prevent violent vibration and prevent the pollen tube from breaking, and observe the germination of pollen under a 60-fold microscope. Some viable pollen has just germinated pollen tubes, and the length is less than the length of the pollen grain itself. times.

[0049] (5) Put the glass slide into the humid box again, and observe it after incubating in the environment...

Embodiment 3

[0055] (1) Put uncracked Lycoris anthers evenly into a dry petri dish with sulfuric acid paper, and place the petri dish in an indoor desiccator for 6 days until the pollen disperses.

[0056] (2) Use a dissecting needle to take an appropriate amount of pollen into a concave glass slide, gently add 2-3 drops of culture solution containing 60 g / L sucrose and 25 mg / L boric acid, and mix gently.

[0057] (3) Gently put the slide glass into a humid box with a relative humidity of 75%, and place it in an environment of 27° C. for 60 min.

[0058] (4) Gently take out the glass slide to prevent violent vibration, in case the pollen tube breaks, and observe the germination of pollen under a 60-fold microscope. It can be seen that some pollen has germinated into a longer pollen tube, and some are still germinating.

[0059] (5) Gently put the concave slide back to the original place to continue culturing. After 30 minutes, observe again under the microscope at 60 times. It can be seen ...

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Abstract

The invention discloses a method for measuring pollen vitality of lycoris plants using an in-vitro germination method, comprising the following steps: collecting pollens and placing the pollens into culture solution to perform in-vitro culture, wherein formula of the culture solution consists of 30-80 g / L of sucrose and 5-25 mg / L of boric acid; the temperature for the in-vitro culture is 25-35 degrees centigrade; the time for the in-vitro culture is 60-90 min; and the relative humidity for the in-vitro culture is 70-90%. According to the invention, in-vitro culture conditions of the pollens of lycoris plants are explored; a simple, rapid and feasible method for measuring the pollen vitality of the lycoris plants is provided; and basis for cross breeding of the lycoris plants is provided.

Description

technical field [0001] The invention relates to the field of measuring plant pollen vigor, in particular to a method for measuring the pollen vigor of Lycoris plants by in vitro germination. Background technique [0002] Amaryllis is a kind of plant with great ornamental value. It has huge flowers and bright colors. Because of its strong resistance to shade, it is mostly planted in the understory, forest edge, and grass edge in landscaping. my country's Lycoris plants are very rich in resources, but most of them are still in the wild state, and the artificial planting species are still very single. Therefore, the breeding of new varieties of Lycoris is helpful to promote the application of Lycoris plants in landscaping. [0003] In the conventional breeding of plants, in order to carry out artificial assisted pollination or cross pollination, it is necessary to collect and store pollen in the early stage, especially in the work of cross breeding, researching the vitality of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 肖郁绵夏宜平常乐佘琳芳
Owner ZHEJIANG UNIV
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