Novel in vitro model of human placental barrier
A human placenta and barrier body technology, applied to embryonic cells, vertebrate cells, animal cells, etc., can solve problems such as umbilical vessel stenosis, easily damaged tissues, and placental permeability that cannot be accurately evaluated by drugs
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[0032] Example
[0033] 1. Preparation of reagents
[0034] 1. KRBB: Weigh out 6.923g NaCl, 0.354g KCl, MgSO 4 ·7H 2 O 0.288g, CaCl 2 ·6H 2 O 0.56g, NaHCO 3 2.1g, Na 2 HPO 4 0.136g, glucose 1.8g, L-alanine 0.178g, after mixing, fully dissolve in 800mL double-distilled water, adjust the PH value to 7.4, make up the volume to 1000mL with double-distilled water, pass through 0.22μm filter membrane, and pack, 4℃ save.
[0035] 2. Cryoprotection solution A: Mix 7.5% ethylene glycol, 7.5% DMSO, and 85% pure water to make a solution.
[0036] 3. Cryoprotection solution B: Mix 15% ethylene glycol, 15% DMSO, and 70% pure water to prepare a solution.
[0037] 2. Establish an in vitro model of human placental barrier
[0038] 1. Preparation of placental tissue slices
[0039] 1) Collect the placenta: Choose the placenta of healthy parturients who are 25-30 years old and give birth at 36-40 weeks gestation. After the placenta is delivered, under aseptic conditions, rinse the placenta 3 times with ...
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