Application of mangiferin compound or mango extraction as pancreatic lipase inhibitor and to preparation of medicament or food for preventing and treating obesity
A technology of pancreatic lipase and mangiferin, which is applied in the directions of medical preparations containing active ingredients, application, food preparation, etc., can solve the problem that the weight loss activity of mangiferin compounds has not been reported.
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[0122] The preparation method of the mango extract is as follows: taking fresh mango fruit or mango leaves, extracting with ethanol solution, and concentrating the obtained extraction solution into an extract to obtain the product.
[0123] The aqueous ethanol solution used for extraction is a mixture of ethanol and water. In order to improve the extraction effect, an aqueous ethanol solution with an ethanol content of 70% (volume fraction) is used.
[0124] The present invention also provides the application of a mangiferin compound with the structure shown in formula I in the preparation of medicine and / or food for treating and / or preventing obesity.
[0125]
[0126] Formula I
[0127] Among them, R 1 selected from -Glucose, -OH or -H;
[0128] R 2 selected from -OH or -OCH 3 ;
[0129] R 3 selected from -Glucose, -OH or -H.
[0130] Among them, the mangiferin compounds with the structure shown in formula I can be commercially available or prepared according to th...
Embodiment 1
[0183] Example 1 Detection of inhibitory activity of commercially available mangiferin compounds on pancreatic lipase
[0184] The internationally recognized pancreatic lipase assay method was used to determine the activity of pancreatic lipase according to the release rate of oleic acid released from triolein. The mangiferin compounds used in the test include: mangiferin, isomangiferin, homomangiferin, mangiferin aglycon, isomangiferin aglycone, and homomangiferin aglycon. All are purchased from the market.
[0185] The detection method is as follows: prepare a solution containing 0.1 mol / L N-tris(hydroxymethyl)methyl-3-aminoethanesulfonic acid (TES) and 0.1 mol / L NaCl, and adjust the pH value to 7.0, which is the detection buffer . Take 80mg of glyceryl trioleate, 10mg of lecithin and 5mg of taurocholic acid, dissolve in 9mL of detection buffer, and ultrasonicate for 5min.
[0186] Take 0.1 mL of the ultrasonically treated suspension, add 0.05 mL of pancrelipase and the s...
Embodiment 2
[0192] Example 2 Effects of Commercially Available Mangiferin Compounds on the Body Weight of High Fat-fed Mice
[0193] The method used in the experiment was as follows: 54 ICR female mice were divided into 3 groups, 18 in each group, and fed with different foods. After 8 weeks, the animals were anesthetized with ether and sacrificed. The body weight of each mouse was measured once every 2 weeks, and the food intake of each mouse was recorded 3 times a week for 8 weeks, and the effect of mango extract or mangiferin compounds on the body weight of mice fed with high fat was analyzed. The mangiferin compounds used in the test include: mangiferin, isomangiferin, homomangiferin, mangiferin aglycon, isomangiferin aglycone, and homomangiferin aglycon.
[0194] The feeding food was as follows: control group, fed with common feed; high-fat food group: feed containing 40% tallow, 36% casein, 10% cornstarch, 9% sugar, 1% mixed vitamins, 4% minerals; experimental group : Add 1% mangife...
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