Method and fermentation culture medium used for synthesizing gamma-aminobutyric acid through microbial fermentation

A technology of microbial fermentation and aminobutyric acid, applied in the field of microbial fermentation and synthesis of γ-aminobutyric acid, method and fermentation medium, can solve the problems of high salt content in the medium, low GABA content, unsafe conditions, etc., and achieve simple preparation, The effect of high output and easy operation

Active Publication Date: 2013-02-13
北京航天恒丰科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] Aiming at the low and unsafe natural food GABA content in the prior art; whil

Method used

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  • Method and fermentation culture medium used for synthesizing gamma-aminobutyric acid through microbial fermentation
  • Method and fermentation culture medium used for synthesizing gamma-aminobutyric acid through microbial fermentation
  • Method and fermentation culture medium used for synthesizing gamma-aminobutyric acid through microbial fermentation

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Experimental program
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Effect test

Embodiment 1

[0055] After the Lactobacillus brevis L2 strain was activated on the MRS culture plate for 24 h, a ring of the strain was transferred to the GYP seed medium, cultured for 24 h as fermented seeds, and inoculated with 10 g / L rice bran fermentation medium, cultured statically at 30°C for about 66 h to 72 h, the lactic acid bacteria fermentation liquid with GABA content of 12.247 g / L can be obtained by HPLC analysis.

Embodiment 2

[0057] After the Lactobacillus brevis L2 strain was activated on the MRS culture plate for 24 h, a ring of the strain was transferred to the GYP seed medium, cultured for 24 h as fermented seeds, and inoculated with 3% (v / v) inoculum to 20 g / L rice bran fermentation medium, cultured statically at 30°C for about 66 h to 72 h, the lactic acid bacteria fermentation liquid with GABA content of 19.134 g / L can be obtained by HPLC analysis.

Embodiment 3

[0059] After the Lactobacillus brevis L2 strain was activated on the MRS culture plate for 24 h, a ring of the strain was transferred to the GYP seed medium, cultured for 24 h as fermented seeds, and inoculated with 3 % (v / v) inoculum containing 30 g / L rice bran fermentation medium, cultured statically at 30°C for about 66 h to 72 h, the lactic acid bacteria fermentation liquid with GABA content of 23.78 g / L can be obtained by HPLC analysis.

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Abstract

The invention relates to a microbial fermentation method, and especially relates to a method and a fermentation culture medium used for synthesizing gamma-aminobutyric acid (GABA) through microbial fermentation. The gamma-aminobutyric acid synthesizing method adopts a microbe which is a lactobacillus brevis L2 strain. The lactobacillus brevis L2 strain is subjected to MRS culturing flat plate activation; an obtained strain is transferred into a GYP seed culture medium; seeds used in fermentation are cultured; and the material is inoculated in the culture medium with an inoculation amount of 1-5% by volume. The culture medium comprises 10-100g/L of millet bran, and 30-80g/L of L-glutamic sodium. An initial pH value of the culture medium is regulated to 3.00-5.00. Static culture is carried out for 50-80h under a temperature of 20-45 DEG C, such that a lactobacillus fermentation broth with high gamma-aminobutyric acid content is obtained. The culture medium provided by the invention is pure natural, and has the advantages of simple preparation and low raw material cost. Biosynthesis of GABA has the advantages of simple operation, high yield, easy separation and purification, and no pollution. A product reaches a food safety level.

Description

technical field [0001] The invention relates to a microbial fermentation method, in particular to a method for synthesizing gamma-aminobutyric acid by microbial fermentation and a fermentation medium. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, GABA for short) is a natural amino acid composed of non-protein, and is the most important inhibitory neurotransmitter widely distributed in the mammalian central nervous system, about 50% of the central synapse The part uses GABA as the transmitter, plays an important role in the cerebral cortex, hippocampus, thalamus, basal ganglia and cerebellum, and has a regulating effect on various functions of the body, so GABA is a good raw material for medical drugs and health products . With the deepening of research, the physiological function of GABA has been continuously elucidated, and it has developed into a new type of functional factor, which is gradually being widely used in medicine, food health care, ch...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12R1/24
Inventor 蒋冬花高爱同
Owner 北京航天恒丰科技股份有限公司
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