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Material sterilization method with combination of continuous sterilization and batch sterilization in fermentation of glutamic acid

A sterilization method and technology for fermenting materials, applied in heating and other directions, can solve the problems of large damage to the medium, high energy consumption, scaling of heat exchangers, etc. Effect

Active Publication Date: 2014-07-23
LINGHUA GROUP INCORPORATED COMPANY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The advantage of continuous sterilization is that the consumption of steam and cooling water can be saved through the heat exchanger. At the same time, since heating and cooling are completed in a very short time, it can reduce the destruction of the medium and improve the quality of the medium. Therefore, for large-volume fermentation Tanks generally use the continuous elimination method; however, there is also a problem that is difficult to solve in the continuous elimination process, that is, some Pellanite components are easy to cause fouling of the heat exchanger, and the workload of cleaning the heat exchanger is huge, which affects the batch of tanks. At the same time, it affects the heat exchange effect and even leads to incomplete sterilization.
The advantage of solid tank sterilization is that the equipment is simple, there is no fouling problem in the heat exchanger, and the sterilization effect is more reliable than continuous sterilization. The damage is large, and at the same time, because there is no heat exchange device, the energy consumption is high

Method used

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  • Material sterilization method with combination of continuous sterilization and batch sterilization in fermentation of glutamic acid
  • Material sterilization method with combination of continuous sterilization and batch sterilization in fermentation of glutamic acid

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Embodiment 1

[0022] Embodiment 1 The material sterilization method that Lianshixiao combines in glutamic acid fermentation,

[0023] (1) Fermentation medium composition: corn steep liquor, glucose, phosphoric acid, magnesium sulfate, potassium chloride and soybean meal hydrolyzate (specifically, the weight ratio of each component of the fermentation medium to the total fermentation material is: corn steep liquor 5%, glucose 4-10%, phosphoric acid 0.2-0.4%, magnesium sulfate 0.1-0.2%, potassium chloride 0.3-0.5%, soybean meal hydrolyzate 0.2%);

[0024] (2) Real consumption: mix corn steep liquor, phosphoric acid, and soybean meal hydrolyzate in the fermentation medium, add ingredient water, control the volume to 10% of the total fermentation material volume, put it into the fermentation tank, and heat up to 123°C and keep for 50 minutes;

[0025] (3) Continuous elimination: mix glucose, magnesium sulfate, and potassium chloride, add ingredient water, heat up to 126°C through the continuou...

Embodiment 2

[0027] Embodiment 2 The material sterilization method that Lianshixiao combines in glutamic acid fermentation,

[0028] 1. Composition of fermentation medium:

[0029] Corn steep liquor: (5%), glucose (4%--10%), phosphoric acid (0.2-0.4%), magnesium sulfate (0.1-0.2%), potassium chloride (0.3%--0.5%), soybean meal hydrolyzate (0.2%);

[0030] 2. Mix corn steep liquor, phosphoric acid, and soybean meal hydrolyzate together, and put them directly into the tank to be sterilized, and add ingredient water to make the volume reach 10% of the total volume.

[0031] 3. The fermenter (including the 2-step mixture) is heated from the bottom by steam, so that the temperature reaches 122°C--125°C and keeps for 40-60 minutes.

[0032] 4. Mix other components except the medium material in step 2 together, and add batching water to make up the volume, raise the temperature to 125°C-128°C through the continuous elimination device, maintain the temperature in the tank for 8-10 minutes, and r...

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Abstract

The invention discloses a material sterilization method with combination of continuous sterilization and batch sterilization in fermentation of glutamic acid. The novelty is reflected by firstly determining the composition of a fermentation culture medium, performing batch sterilization and continuous sterilization and further performing inoculation and fermentation. A continuous sterilization heat exchanger adopting the technology does not need to perform washing frequently; the fermentation can further prevent pollution, and the individual disinfection effect is even better; and the consumption of both steam and cooling water can be significantly reduced.

Description

technical field [0001] The invention belongs to the technical field of glutamic acid fermentation production, and in particular relates to the technology of culture medium sterilization. Background technique [0002] The glutamic acid fermentation process requires high-temperature sterilization of the culture medium. Generally, there are two methods of sterilization: one is solid tank sterilization, that is, the prepared medium is pre-filled into the fermentation tank (or seed tank), The temperature is raised in the tank, and after reaching the required temperature, it is maintained for a certain period of time, and the temperature is lowered to the temperature required for fermentation to inoculate and ferment. The second is continuous sterilization, that is, the fermenter (or seed tank) is pre-empty and sterilized, and then the prepared medium is heated through a continuous sterilizer, the temperature of the tank is maintained for a certain period of time, and the temperat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/07
Inventor 杨玉岭岳希金满德恩李高卫郭脉海李国良
Owner LINGHUA GROUP INCORPORATED COMPANY
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