Formula of eyesight protecting heath care product and preparation method thereof
A health care product and formula technology, applied in the field of medicine, can solve the problems of food or medicine that have not been developed for eye care and health care, and achieve the effects of protecting eyes, improving blood circulation and improving flow.
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Embodiment 1
[0019] (1) Take gardenia fruit and crush it into coarse powder, add 10 times of 40% ethanol to percolate for 24 hours, filter, and concentrate the percolate until it has no alcohol smell;
[0020] (2) Take the concentrated solution of step (1) directly or dilute it with water and then use macroporous adsorption resin HPD-100 to separate and purify;
[0021] (3) Concentrate the product of step (2) with 10 times the volume of pure water and 10% ethanol eluent, combine the 80% ethanol eluent, concentrate and recover ethanol, and obtain crocin after freeze-drying;
[0022] (4) Mix the crocin and anthocyanin obtained in step (3) to make a vision protection product. In this embodiment, the weight ratio of crocin and anthocyanin is 1:1.
[0023] Medication trials of vision care products:
[0024] Human retinal pigment epithelial cells were cultured and the light intensity was adjusted to 2500 lx. When the retinal pigment epithelial cells in the 96-well plate reached 80% confluency...
Embodiment 2
[0039] (1) Take gardenia fruit and crush it into coarse powder, add 10 times of 40% ethanol to percolate for 24 hours, filter, and concentrate the percolate until it has no alcohol smell;
[0040] (2) Take the concentrated solution of step (1) directly or dilute it with water and then use macroporous adsorption resin HPD-100 to separate and purify;
[0041] (3) Concentrate the product of step (2) with 10 times the volume of pure water and 20% ethanol eluent, combine the 60% ethanol eluent, concentrate and recover ethanol, and obtain crocin after freeze-drying;
[0042] (4) Mix the crocin and anthocyanin obtained in step (3) to make a vision protection product. In this embodiment, the weight ratio of crocin and anthocyanin is 9:1.
Embodiment 3
[0044] (1) Take gardenia fruit and crush it into coarse powder, add 10 times of 40% ethanol to percolate for 24 hours, filter, and concentrate the percolate until it has no alcohol smell;
[0045] (2) Take the concentrated solution of step (1) directly or dilute it with water and then use macroporous adsorption resin HPD-100 to separate and purify;
[0046] (3) Concentrate the product of step (2) with 10 times the volume of pure water and 30% ethanol eluent, combine the 40% ethanol eluent, concentrate and recover ethanol, and obtain crocin after freeze-drying;
[0047] (4) Mix the crocin and anthocyanin obtained in step (3) to make a vision protection product. In this embodiment, the weight ratio of crocin and anthocyanin is 1:9.
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