Novel technology method for purifying berberine in wild berberis sibirica

Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for extracting and purifying berberine from wild berberis roots in great khingan, in particular to a novel method for purifying berberis by a biological enzymolysis, ultrasonic extraction and column chromatography purification combined technique. The method has the advantages that 1, a biological enzymolysis technique is applied to allow that berberine in cells is dissolved out fully; 2, an ultrasonic extraction technique is adopted; the heating time for an effective component is shortened; the activity loss is less; the bioactivity of a product is high; and 3, a silica gel column chromatography and recrystallization purification technique is adopted, so that the purity of the effective component, namely berberine is further increased; the operation is simple and easy; the production cost is lowered greatly; and the method can be applicable to industrial use. According to the method, the biological enzymolysis technique is adopted, the effective component is dissolved out as much as possible, then ultrasonic extraction is conducted, alcohol serves as a solvent, then column chromatography purification is conducted, and finally recrystallization is conducted for secondary purification, so that the purity of berberine is increased to 98.4%.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting and purifying berberine from three wild needles in Daxing'an Mountains, in particular to a method for purifying berberine by using biological enzymolysis, ultrasonic extraction combined with column chromatography purification technology new method. Background technique: [0002] Berberis spinosa, also known as the Northeast Three Needles, is a small deciduous shrub that grows on gravel hillsides at high altitudes. It is produced in Dabald Mountain and Tahe Forestry Bureau of Daxinganling Debuer Forestry Bureau. up to 100 cm. The bark is dark gray-black, with thin longitudinal grooves on the branches; the branches are dark gray-brown, with 5-8 forked thorns at the base of the short branches; the thorns at the lower part of the branches are more forked, thin and sharp, dark gray. Leaves clustered on short branches, obovate, oblanceolate or obovate-...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages of conventional techniques such as low extraction rate and low product purity, and provide a method for utilizing biological enzymolysis, ultrasonic intensified extraction and performing silica gel column chromatography to purify three needles of berberine, in order to achieve the above object , the technical scheme that the present invention adopts is: a kind of new method that utilizes biological enzymatic hydrolysis coupled ultrasonic wave to strengthen extraction, and then combines column chromatography to purify berberine, and its steps are as follows: