Method for simultaneously detecting phthalic acid esters (PAEs) environmental hormone and metabolite in water sample
A technology for metabolites and environmental hormones, applied in the field of simultaneous quantitative detection of PAEs-like environmental hormones and their metabolites in water samples, can solve the problem of difficulty in simultaneously detecting PAEs-like environmental hormones and their metabolites, etc., to avoid interference and ensure correctness effect
Inactive Publication Date: 2014-08-13
CHINA JILIANG UNIV
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[0006] Existing methods for detecting PAEs class environmental hormones include HPLC, GC, GC-MS and LC-MS / MS methods, methods for detecting PAEs class environmental hormone metabolites in the environment include HPLC method, LC-MS / MS method, these It is difficult to detect PAEs environmental hormones and their metabolites simultaneously
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[0026] The present invention will be further described below in conjunction with accompanying drawing.
[0027] Such as figure 1 Shown are the standard chromatograms of three PAEs environmental hormones and their metabolites; figure 2 Shown is the detection chromatogram of PAEs environmental hormones and metabolites thereof in the water sample of the present embodiment; Wherein: peak 1 is mDBP chromatographic peak, peak 2 is DBP chromatographic peak, peak 3 is mBBP chromatographic peak; Peak 4 is mDEHP Chromatographic peak; peak 5 is BBP chromatographic peak; peak 6 is DEHP chromatographic peak.
[0028] In the simultaneous quantitative detection method of PAEs environmental hormones and their metabolites in water samples of the present invention, the liquid water sample to be tested is carried out in sequence by the following steps:
[0029] 1) Add 100% pure dibutyl phthalate (DBP), butyl benzyl phthalate (BBP), diethylhexyl phthalate (DEHP) and its Corresponding metaboli...
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The invention discloses a method for simultaneously detecting phthalic acid esters (PAEs) environmental hormone and metabolite thereof in a water sample. The method comprises the following steps: gas chromatography-Fisher identification (GC-FI) analysis is performed on a reference substance and a liquid layer which is obtained by processing to obtain the peak areas of the PAEs environmental hormone and the metabolite thereof in the reference substance and the liquid layer respectively; according to the computational formula that the contents of the PAEs environmental hormone and the metabolite thereof in the to-be-detected water sample is equal to the quotient of the product of the peak areas of the PAEs environmental hormone and the metabolite thereof in the liquid layer and the concentration of the PAEs environmental hormone and the metabolite thereof in the reference substances and the peak areas of the PAEs environmental hormone and the metabolite thereof in the reference substance, so that the content of the PAEs environmental hormone and the metabolite thereof in the to-be-detected water sample is obtained. The method for simultaneously and quantitatively detecting the PAEs environmental hormone and the metabolite thereof in water environment has the advantages of high accuracy and high sensitivity.
Description
technical field [0001] The invention relates to a method for detecting environmental hormones, in particular to a simultaneous quantitative detection method for PAEs environmental hormones and their metabolites in water samples. Background technique [0002] Phthalic acid esters (Phthalic Acid Esters, referred to as PAEs, alias phthalates) are an important class of organic compounds, mainly used in the production of plastic modification additives, pesticides, coatings, printing and dyeing, cosmetics, paints and fragrances. In recent years, studies have found that PAEs compounds have estrogen-like activity, and PAEs have been classified as environmental hormones (also known as environmental hormones or endocrine disruptors), which can affect the normal behavior of organisms and the synthesis of normal hormones related to reproduction and development. The processes of storage, secretion, transport in vivo, combination and clearance create obstacles, so the accumulation and mig...
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IPC IPC(8): G01N30/02
Inventor 葛建胡天宇胡华军韩宝瑜姚元发王敏君屈叶青王慧袁萍李燕冷贞贞杨丛军刘晓菲
Owner CHINA JILIANG UNIV