A kind of nucleic acid and its application and pharmaceutical composition
A composition and nucleic acid technology, applied in the direction of introducing foreign genetic material, medical formula, genetic material components, etc. by using a carrier, which can solve problems such as poor effect
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[0036] According to a particularly preferred embodiment of the present invention, the nucleic acid contains siRNA(m)-8 whose sense strand sequence is SEQ ID NO: 78 and antisense strand sequence is SEQ ID NO: 79, and the sense strand sequence is SEQ ID NO: 80 And the antisense strand sequence is siRNA(m)-23 of SEQ ID NO:81, the sense strand sequence is SEQ ID NO:82 and the antisense strand sequence is siRNA(m)-27 and the sense strand sequence of SEQ ID NO:83 It is SEQ ID NO: 84 and the antisense strand sequence is siRNA(m)-30 of SEQ ID NO: 85, the sense strand sequence is SEQ ID NO: 86 and the antisense strand sequence is siRNA (m) of SEQ ID NO: 87 -32 and at least one of siRNA(m)-34 whose sense strand sequence is SEQ ID NO: 88 and antisense strand sequence is SEQ ID NO: 89.
[0037] According to the nucleic acid of the present invention, wherein the siRNA comprising the siRNA-8, the siRNA-23, the siRNA-27, the siRNA-30, the siRNA-32 and the siRNA-34 can be passed Obtained by ...
preparation Embodiment 1
[0052] The siRNAs listed in Table 1 were chemically synthesized by Shanghai GenePharma Company.
[0053] Table 1
[0054]
[0055]
[0056] After sequence comparison, there is no homologous sequence with a length of more than 16 bases between the sequence of the above siRNA and the genome sequence of human or mouse. Moreover, the above siRNA is formed by annealing equimolar sense strand and antisense strand.
preparation Embodiment 2
[0058] Entrust Shanghai GenePharma Company to prepare plasmids expressing shRNA listed in Table 2. The plasmid expressing shRNA is recorded as shRNA(p), which are shRNA(p)-8, shRNA(p)-23, shRNA(p)-27, shRNA(p)-30, shRNA(p)-32, shRNA( p)-34, shRNA(p)-4, shRNA(p)-9, shRNA(p)-17, shRNA(p)-22, shRNA(p)-26, shRNA(p)-35, shRNA(p) )-36, shRNA(p)-37, shRNA(p)-38, shRNA(p)-40 and shRNA(p)-45. Among them, all plasmids expressing shRNA are inserted into the empty vector numbered pGenesil-1 purchased from Wuhan Jingsai Company (the sequence of the empty vector is as shown in its instruction manual), and a positive-sense short reverse sequence corresponding to the positive-sense strand of siRNA is inserted. A nucleic acid fragment encoding shRNA composed of a repeat fragment, a loop fragment (SEQ ID NO: 25, namely 5'-TCAAGAGA-3') and an antisense short inverted repeat fragment corresponding to the antisense strand of siRNA concatenated.
[0059] Table 2
[0060]
PUM
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