Puer tea immune adjuvant and application thereof in influenza vaccines

A technology for immune adjuvant and influenza vaccine, which is applied to the immune adjuvant of Pu'er tea, the application field of Pu'er tea extract in the preparation of immune adjuvant, and the immune adjuvant of influenza vaccine, which can solve the problem of limited supply of vaccine production and effective vaccine effect. Slow time, unavoidable side effects and other problems, to achieve the effect of improving immunogenicity, enhancing immune effect, and good effectiveness

Active Publication Date: 2013-05-08
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In order to solve the current single immune adjuvant, side effects are unavoidable, vaccine production and supply are limited, it is difficult to meet the urgent need to provide a large number of vaccines when an epidemic breaks out, and the onset time of the vaccine Slow and many other problems, the present invention aims to provide a Pu-erh tea immune adjuvant that can effectively improve the immune effect of vaccines and reduce the dosage of vaccine antigens

Method used

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  • Puer tea immune adjuvant and application thereof in influenza vaccines
  • Puer tea immune adjuvant and application thereof in influenza vaccines
  • Puer tea immune adjuvant and application thereof in influenza vaccines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation of Pu'er tea ethanol extract: Take 50g of Pu'er tea, grind it into powder, put it in a container, add 500ml of ethanol with a mass concentration of 95%, and heat and reflux extraction as usual, wherein, each time the heating temperature is 50 ° C, reflux The time is 60 minutes, and the number of extractions is 2 times. After filtration, the filtrate is decompressed to recover ethanol, and the remaining filtrate is concentrated and dried conventionally to obtain an extract.

Embodiment 2

[0051] Preparation of Pu'er tea water extract: Take 100g of Pu'er tea, crush it into powder, put it in a container, add 2000ml of distilled water and heat and decoct it according to the routine to extract, wherein, the decoction time is 4 hours each time, the number of extractions is 3 times, filter After combining the filtrates, the filtrates were concentrated and dried conventionally to obtain extracts.

Embodiment 3

[0053] Preparation of nanoemulsion carrier:

[0054] 1) Prepare materials according to the following mass ratio: 1g of polyoxyethylene castor oil, 1g of Span 80, 1.5g of 1,2-propylene glycol, 0.5g of soybean oil for injection, 0.6g of water for injection;

[0055] 2) Add 1g of polyoxyethylene castor oil, 1g of Span 80, 1.5g of 1,2-propanediol, 0.5g of soybean oil for injection into the mixing bottle, keep stirring for 2 minutes at room temperature, and mix well to obtain a mixture;

[0056] 3) Slowly drop 0.6 g of water for injection into the mixture of the above 2), and at the same time, stir the mixture at a rotation speed of 400 rpm at room temperature until the liquid is transparent to obtain a nanoemulsion carrier;

[0057] 4) The nanoemulsion prepared in 3) was tested as follows: 1. The average particle size was 31.83nm after detection by the Malvern particle size detector, and it was qualified; 2. After centrifugation at 10,000 rpm for 30 minutes, the nanoemulsion syste...

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Abstract

The invention provides a Puer tea immune adjuvant. The adjuvant is characterized by consisting of the following components in percentage by mass: 0.05 to 100 percent of Puer tea extract and 0 to 99.95 percent of a carrier. The influenza vaccine adjuvant prepared from the Puer tea extract has good effectiveness, can improve the immunogenicity of antigens and enhance the immune effect, and solves numerous problems that the conventional immune adjuvant is single, the side effect is difficult to avoid, production and supply of vaccines are limited, the requirement for urgently providing a large quantity of vaccines in epidemic outbreak is difficult to meet, the response time of the vaccines is long and the like; and the quality of the vaccines is easy to control. A preparation method for the Puer tea extract is simple, convenient, wide in material source, economic and environment-friendly.

Description

technical field [0001] The invention relates to a Pu'er tea immune adjuvant, in particular to the application of a Pu'er tea extract in preparing an immune adjuvant, especially an immune adjuvant for influenza vaccine, and belongs to the field of biotechnology. technical background [0002] Pu'er tea is originally produced in Simao and Xishuangbanna, Yunnan, my country. It is a famous historical tea. It is processed with fresh leaves of Yunnan big-leaf tea tree as raw material. According to different fermentation methods, it is divided into raw tea and cooked tea, namely Yunnan big-leaf tea After the fresh tea leaves from the tea tree are picked, the raw tea is obtained by sun drying, and then the raw tea is slowly and naturally fermented, or the cooked tea is fermented artificially. Pu-erh tea has loose tea and pressed shaped tea, and has the following unique quality characteristics: the color of cooked tea is brownish red, the soup color is brownish red, clear, and has a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/145A61P37/04A61P31/16
Inventor 李映波刘洪卿孙笠雯赵兰华杨磊
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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