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Artificial cartilage and its production method

A manufacturing method and cartilage technology, which can be used in bone diseases, medical science, prostheses, etc., can solve the problem of no compounding, and achieve the effects of excellent self-organization, simple manufacturing, and excellent biological affinity.

Inactive Publication Date: 2013-05-08
HOYA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the complex obtained by the method described in International Publication No. 2007 / 032404 actually consists of collagen components only, and sufficient complexation has not occurred

Method used

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  • Artificial cartilage and its production method
  • Artificial cartilage and its production method
  • Artificial cartilage and its production method

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Experimental program
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preparation example Construction

[0035] (A) Preparation of the first and second compositions

[0036] In the process of preparing the first composition, the mixing ratio (mass ratio) of hyaluronic acid and collagen is preferably 10000:1 to 1:10000, more preferably 5000:1 to 1:5000, and most preferably 15:1 to 1 : 15. It is preferable to use a substance obtained by pre-dissolving collagen in dilute hydrochloric acid (a concentration of about 5 to 50 mM) at a concentration of 0.1 to 20% by mass. In addition, it is preferable to use a substance obtained by dissolving hyaluronic acid in sterile water (water for injection, etc.) at a concentration of 0.1 to 20% by mass. It is preferable to mix the hyaluronic acid aqueous solution and the collagen aqueous solution at 3-25°C.

[0037] In the step of preparing the second composition, the mixing ratio (mass ratio) of proteoglycan and collagen is preferably 10000:1 to 1:10000, more preferably 5000:1 to 1:5000, most preferably 10:1 to 1 : 10. It is preferable to use a su...

Embodiment 1

[0072] (1) Preparation of raw material solution

[0073] The collagen was dissolved in 5 mM hydrochloric acid to prepare an aqueous collagen solution with a concentration of 1% by mass. In addition, proteoglycan was dissolved in water for injection to prepare an aqueous solution of proteoglycan with a concentration of 1% by mass. In addition, hyaluronic acid was dissolved in water for injection to prepare an aqueous hyaluronic acid solution with a concentration of 0.1% by mass. In addition, these preparations were all performed at 4°C.

[0074] (2) Mixing of raw materials

[0075] Mix the above-mentioned collagen aqueous solution and proteoglycan aqueous solution at 1:1 (mass ratio), and stir with a mixer to obtain a mixed solution A. Similarly, the above-mentioned collagen aqueous solution and the hyaluronic acid aqueous solution were mixed at 1:1 (mass ratio), and stirred with a mixer to obtain a mixed solution B. Mix the mixtures A and B at 2:1 (mass ratio), and stir for 1 min...

Embodiment 2

[0087] (1) Preparation of raw material solution

[0088] The collagen was dissolved in 5 mM hydrochloric acid to prepare an aqueous collagen solution with a concentration of 1% by mass. Proteoglycan was dissolved in water for injection to prepare an aqueous solution of proteoglycan with a concentration of 1% by mass. In addition, hyaluronic acid was dissolved in water for injection to prepare an aqueous solution of hyaluronic acid with a concentration of 0.2% by mass. In addition, these preparations were all performed at 4°C.

[0089] (2) Mixing of raw materials

[0090] 22.5 mL of the prepared collagen aqueous solution, 105 mL of the proteoglycan aqueous solution, and 112.5 mL of the hyaluronic acid aqueous solution were mixed, and stirred for 1 minute with a homogenizer at a rotation speed of 2,000 rpm. Stirring was performed while keeping the temperature of the sample at 5°C.

[0091] (3) Freeze drying

[0092] The obtained mixture was grouted in a tray, frozen at -80°C for 12 ho...

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Abstract

An artificial cartilage comprising 15-95% by mass of collagen, 4.9-70% by mass of proteoglycan, and 0.1-20% by mass of hyaluronic acid.

Description

Technical field [0001] The invention relates to artificial cartilage and a manufacturing method thereof. The present invention relates to elastic artificial cartilage using components of biological cartilage as a raw material, and a method for producing the same. Background technique [0002] Cartilage tissue is composed of cartilage cells and cartilage matrix (matrix). Chondrocytes are highly differentiated cells, which account for only about 10% of cartilage tissue. Although there is basically no proliferation through cell division, chondrocytes produce cartilage matrix components in cartilage tissue, which helps maintain the cartilage tissue accounted for about 90%. % Cartilage matrix. [0003] Although attempts have been made to artificially regenerate cartilage tissue using chondrocytes and use them in treatments for cartilage damage and degeneration, in order to form cartilage-like tissue, the process of making cartilage cells themselves produce cartilage matrix components ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/22A61L27/20
CPCA61L27/24A61L27/26A61L27/56A61L2430/06A61F2/02A61P19/04C08L89/06C08L5/08C08L89/00
Inventor 河东万智子
Owner HOYA CORP
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