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Swirl-based single-cell posture adjustment method

A regulation method, single-cell technology, applied in biochemical equipment and methods, bioreactors/fermenters for specific purposes, biochemical instruments, etc., to achieve the effect of improving versatility and precise regulation

Inactive Publication Date: 2013-05-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the two jet tubes are fixed in the horizontal plane, the requirement for the cell size is fixed, and the positioning operation before the cell attitude adjustment is difficult
It can be seen that in the existing microfluidic-based cell posture adjustment methods, generally only for cells with a fixed size and shape and a certain viscosity of the culture medium, when these parameters change, the driving and control parameters of the microfluidics are all different. Had to be retuned, maybe even redesigned

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Embodiment Construction

[0022] The specific implementation of the present invention will be further introduced below in conjunction with the accompanying drawings, but the implementation and protection scope of the present invention are not limited thereto.

[0023] The single-cell attitude adjustment method based on swirling flow mainly includes the following steps to achieve cell attitude adjustment:

[0024] (1) if figure 1 , according to the shape and size of the cell 3, make the cell in the symmetrical center position of the first moving microtube 1 and the second moving microtube 2, and adjust the ratio of the distance a between the end faces of the two microtubes to the diameter b of the cell 3 as: 1 :1~3:1.

[0025] (2) Keep the distance a between the ends of the two microtubes (1 and 2) unchanged, and adjust the distance b between the inner diameters of the two microtubes (1 and 2) to be consistent with the distance a between the ends of the two microtubes (1 and 2), even if a=b; in step ...

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Abstract

The invention provides a swirl-based single-cell posture adjustment method. The method comprises the steps of in the operation process of cell attitude adjustment, adjusting the end surface distance and the axial distance of two parallel microtubes according to the shape and size of cell; controlling the two microtubes to spray fluids at the same time to generate swirl; and driving the cell to rotate by using the viscous force of the swirl, and realizing adjustment of any angle of the cell through a pulse spraying mode. The method provided by the invention can adapt to different changes of the cell shape and size and the solution viscosity, and accurately rotate the cell to achieve the expected posture, cannot cause mechanical damage of the cell and is favorable for improving the success rate of the cell experiment operation.

Description

technical field [0001] The present invention relates to the technical field of single-cell attitude adjustment, in particular to a single-cell attitude adjustment method based on swirling flow. Background technique [0002] Cell posture regulation is a difficult problem in bioengineering. At present, the practical operation method of cell posture adjustment is to use microneedles to fiddle with cells to make them rotate, which has problems such as large damage to cells, low precision, low repeatability, high cost, and high labor intensity, which greatly restricts micromanipulation. Popularization and application of technology. Researchers generally hope to replace manual work with machinery, and replace manual work with automation, so that micromanipulation technology can be simplified and automated, and then engineering can be realized. [0003] The direct contact method of cell attitude adjustment will damage the cells, affect the success rate of the experiment, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
Inventor 黄维军张勤
Owner SOUTH CHINA UNIV OF TECH