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Application of a kind of pyrogallic acid in inhibiting the growth of algae

A technology of pyrogallic acid and biomass, applied in application, plant growth regulators, chemicals for biological control, etc., can solve the problems of increased difficulty, heavy workload, short duration of effect, etc., and achieve ecological safety good sex effect

Active Publication Date: 2014-10-15
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, physical removal mainly includes mechanical or manual salvage, clay flocculation and other methods, which are suitable for emergency removal of algal blooms, but the workload is heavy, the cost is high, and further disposal of collected algae is faced, so it is not suitable for large-scale algal blooms. Eradication; chemical algae killing is mainly to apply a certain amount of chemical agents to the algae waters. This type of method is easy to operate and has quick results, but the effect lasts for a short time. Its decomposition products will inevitably cause secondary pollution and cannot be continuously used in large areas; biological algae control technology mainly uses organisms located in the upper food chain such as silver carp and bighead carp to feed on algal blooms or use microorganisms to control algal blooms. Flocculation or lysis of algal cells can achieve the purpose of eliminating algal blooms, but for toxic algal blooms, facing the bioaccumulation of toxins in the food chain or the release of toxins in large quantities in water bodies, algae control will also bring new ecology safe question
[0004] In addition, the use of algicides is only a palliative, not a permanent cure. Once the algicide occurs, even if the algicide is used, the algae will be killed in a short time, and then the flocculant will be used to sink the algae to the bottom of the water, which can make the water body in a short time. It reaches a transparent state, but due to the existence of nutritional conditions, the temperature rises, the dormant spores will recover, and the water bloom will break out again
At the same time, directly killing cyanobacteria can easily release intracellular algae toxins and other metabolites into the water body due to the destruction of cyanobacteria cells, promote their degradation, and form odorous substances, which instead increases the chances of eliminating these chemicals from the water body and improving water quality. difficulty

Method used

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  • Application of a kind of pyrogallic acid in inhibiting the growth of algae
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  • Application of a kind of pyrogallic acid in inhibiting the growth of algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Inhibition experiment of high-density culture algae:

[0023] The experiment was carried out in a 250mL Erlenmeyer flask, and the vigorous long-term Microcystis aeruginosa was inoculated into 100mL BG11 culture medium, so that the initial algal cell number was 1.0×10 6 cells / ml. Set up three experimental groups and one control group (without algastatic agent), add pyrogallic acid 0.5mg / L, 7.5m / L, and 10mg / L to the experimental groups respectively, and the dosage frequency is once, and then count every day growth of algae. After adding pyrogallic acid, the growth of algae was inhibited to varying degrees. 10mg / L pyrogallic acid treatment, the culture medium remained clear, and the algae growth inhibition rate reached 99.08% ( figure 1 ),From figure 1 It can be seen that after 10mg / L treatment, the whole growth process of algae is in a state of complete inhibition, showing the good algae inhibition effect of pyrogallic acid.

Embodiment 2

[0025] MTT method cell viability detection experiment:

[0026] Microcystis aeruginosa in the logarithmic growth phase was inoculated into 100mL BG11 culture medium, so that the number of initial algal cells was 1.0×10 6 cells / mL or so. Set up two experimental groups and one control group. The experimental groups were inoculated with 2mg / L, 10m / L pyrogallic acid at the same time, and the dosing frequency was one time. active effect ( figure 2 ). It can be seen from the figure that after being treated with pyrogallic acid at 2 mg / L for 4 hours, the vitality of Microcystis aeruginosa cells was reduced to 80.23% of that of the control group, and with the prolongation of the exposure time, the cell viability further decreased , after 24 hours of treatment, the cell viability of the 2mg / L group was reduced to 64.15% of the control, and the result of 10mg / L was reduced to 26.63% of the control. Allelochemicals can rapidly reduce the density of algae in a short period of time. ...

Embodiment 3

[0028] Simulated field addition experiment:

[0029] Under laboratory conditions, maintain sufficient nutritional conditions and light, take Microcystis aeruginosa in the logarithmic growth phase as the algae species, and the initial inoculation concentration is 20,000 cells / L. The experiment has a control, and three exposure concentrations: 0.2mg / L, 0.5mg / L, 1mg / L. According to the dosage method when used in the field, add once a day for 10 consecutive days, and then add once every other week. see results image 3 According to this addition method, after 17 days of culture observation, the growth inhibition rate of the 0.5mg / L and 1mg / L groups reached 94.6%, 95.7%, the algae cell density was lower than 20000 cells / L, and the water bloom remained at no outbreak s level. At the same time, taking the green alga Ceratocystis clematis as a reference, according to the same addition method, the observation results showed that different concentrations of pyrogallic acid could not ...

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Abstract

The invention discloses application of pyrogallic acid in restraining algae growth. The pyrogallic acid is a secondary metabolite naturally secreted from an aquatic plant; the pyrogallic acid or a chemosynthetic compound with a same structure can not influence other organisms in the environment. The usage method is characterized in that the pyrogallic acid is added to a water body before algae blooms erupt and exerts restraining action when algae just starts to grow so that the algae are kept at low density, so that the eruption of the blue algae water blooms of the water body is effectively prevented. The pyrogallic acid has the characteristics of safety, high efficiency, degradability and the like and can be widely applied to the nature or landscape water body.

Description

technical field [0001] The invention belongs to the technical field of ecological algae control, and relates to the application of pyrogallic acid in inhibiting the growth of algae. The allelochemical pyrogallic acid secreted by submerged plants is used to control algae, and by causing damage to algae cell membranes and DNA, Inhibiting the growth of algae can effectively inhibit the outbreak of algal blooms. Background technique [0002] The cyanobacteria bloom caused by the eutrophication of the water body destroys the water body ecosystem, and the algae toxins produced by the algae seriously endanger the water body ecosystem and human safety. How to effectively control the growth of algae in the eutrophic water body and prevent the water bloom from becoming It is one of the important issues that cannot be avoided in ecological protection and restoration of polluted water bodies. [0003] The existing algae control technology can be divided into three categories: physical,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N31/16A01P1/00C02F1/50
Inventor 吴振斌鲁志营郭伟杰刘碧云贺锋周巧红张甬元
Owner INST OF AQUATIC LIFE ACAD SINICA
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