Culture substrate

A technology for cultivating substrates and cultures, which is applied in the direction of tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., which can solve the problems of uneven size and achieve the effect of effective spheroid culture

Active Publication Date: 2013-05-22
AGC TECHNO GLASS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, when the above-mentioned conventional culture substrate 101 is used for spheroid culture, it is found that there are problems as follows: in addition to the spheroid 60, a large number of monolayer cultured cells 62 and spheroids of non-uniform size are formed.

Method used

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no. 1 Embodiment approach

[0028] use Figure 1 to Figure 6 The culture substrate of the first embodiment of the present invention will be described.

[0029] figure 1 It is a perspective view of a culture container having the culture substrate of the present invention. figure 2 It is a longitudinal sectional view of a culture container. image 3 It is a partial perspective view of the pore formation region of the culture substrate. Figure 4 Yes image 3 Section view of IV-IV direction. Figure 5 Yes image 3 The V-V direction view sectional view. Image 6 It is a plan view showing the irradiation spot of the irradiated laser light on the surface of the culture substrate.

[0030] The culture substrate 1 of the present embodiment is a main part of a culture vessel for producing a spheroid (cell aggregate) formed by culturing cells and three-dimensionally aggregating them.

[0031] First, the characteristics of the culture substrate 1 of the present embodiment will be described.

[0032] like...

no. 2 Embodiment approach

[0056] use Figure 7 The culture substrate of the first embodiment of the present invention will be described. Figure 7 It is a partial sectional view of the pore formation region of the culture substrate. It should be noted that the present embodiment is a modified example of the first embodiment, and the same or similar parts as those of the first embodiment are assigned the same reference numerals, and repeated description thereof will be omitted.

[0057] In the first embodiment, the plurality of dimples 20 and banks 22 are formed by irradiating the surface of the culture substrate with laser light. On the other hand, in the present embodiment, the culture substrate 1 is obtained by injection-molding a synthetic resin material using a mold having a convex portion for forming the plurality of dimples 20 and a concave portion for forming the bank portion 22 . The plurality of dimples 20 and dams 22 are formed simultaneously with the molding of the culture substrate 1 .

...

no. 3 Embodiment approach

[0060] use Figure 8 The culture substrate of the first embodiment of the present invention will be described. Figure 8 It is a longitudinal sectional view of a state in which a culture substrate is set in a petri dish. It should be noted that the present embodiment is a modified example of the first embodiment, and the same or similar parts as those of the first embodiment are assigned the same reference numerals, and repeated description thereof will be omitted.

[0061] In the culture substrate 1 of the first embodiment, a plurality of wells 20 are formed on the inner bottom surface (the upper surface of the bottom plate 14 ) of the culture container. On the other hand, the culture substrate 1 of the present embodiment is formed of a disc-shaped synthetic resin material. In the culture substrate 1 , a plurality of dimples 20 are formed on one surface of the culture substrate 1 by injecting a synthetic resin material into a disk-shaped substrate and then irradiating one s...

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Abstract

To efficiently carry out spheroid culturing. A culture substrate is a culture container to culture cells and three-dimensionally aggregate them to prepare spheroids. The culture substrate is made of a synthetic resin material. On a surface of the culture substrate, a plurality of dents 20 to be compartments in which cells are cultured are formed. The plurality of dents 20 are formed by laser irradiation on the culture substrate surface. At the peripheries of openings of the dents 20, the synthetic resin material is melted and piled up to form banks 22. The culture substrate surface between two adjacent dents 20 is a non-flat surface.

Description

technical field [0001] The invention relates to a culture substrate for culturing cultured objects such as cells and tissue pieces to produce spheroids. Background technique [0002] In recent years, attention has been paid to spheroid culture in which cells are cultured and three-dimensionally aggregated instead of monolayer culture in which cells are cultured two-dimensionally. Compared with monolayer culture, spheroid culture can construct a state close to the cells in the living body, and can induce the specific functions of the cells in the living body. [0003] As an example of a conventional culture substrate 101 for culturing spheroids, for example, Figure 11 container shown. like Figure 12 As shown, the bottom surface 114 of the container is formed with a plurality of recessed portions 120 spaced apart from each other. The bottom surface 114 of the container is coated with a cell adhesion inhibitor (not shown) (see Patent Document 1). [0004] In the spheroid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M23/10C12M23/12
Inventor 伊藤亨多田丰和田绫
Owner AGC TECHNO GLASS
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