Methods and compositions for analyte detection

An analyte, target analyte technology, used in the provision of a system for detecting an analyte, a method of binding moieties to detect one or more analytes. , detecting one or more analyte domains

Active Publication Date: 2013-06-12
NEXUS DX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such techniques still suffer from problems encountered in rapid assays designed to detect multiple target analytes

Method used

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  • Methods and compositions for analyte detection
  • Methods and compositions for analyte detection
  • Methods and compositions for analyte detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0361] Example 1. Detection of influenza during the 2007 Australian flu season

[0362] During the 2007 Australian influenza season, a set of 121 nasopharyngeal swab samples were collected. Following nasal sample collection, swabs were placed in 1 mL of viral transport medium and mixed vigorously for 1 min according to standard protocols, then an aliquot was removed for incubation, and the remainder of the sample was frozen. For this test, a swab is dipped into the remaining sample and assayed using the fluID test. An additional 100 μL aliquot was taken from each sample, nucleic acid was purified, and a real-time PCR assay for influenza A virus detection was run.

[0363] Table 6. Results of a study using the 4-line pRNA capture system to detect influenza A

[0364]

[0365] Of the 5 culture- / fluID+ samples, 3 samples were confirmed positive (based on real-time results). When these results were taken into account, the sensitivity, specificity, positive predictive value (...

Embodiment 2

[0367] Example 2. Seasonality determination using titrated cultured virus

[0368] This study examined the analytical performance of A and B analytes in a seasonal assay using titrated cultured virus. Each strain of virus had a TCID50 titer and was individually diluted until no signal was generated in the assay. Each dilution was tested using commercially available point-of-care influenza A and B assay kits and PCR tests. In one embodiment, the dilution sensitivity results show that the A and B analytes are 2-3 logs more sensitive compared to commercially available influenza A and B point-of-care assays, simultaneously compared to PCR In contrast, only 1-2 log is less sensitive.

Embodiment 3

[0369] Example 3. Examination of virus titer levels in infected patients

[0370] This study checks with Quidel QuickVue The analytical performance of the rapid influenza test using the system of the present invention was compared with the system as well as the PCR assay. A and B analytes from different geographic locations were determined. Each strain of virus had a TCID50 titer and was individually diluted until no signal was generated in the assay. Each dilution uses a commercially available Quidel QuickVue Kits as well as PCR tests are tested. Dilution sensitivity studies showed that the system of the present invention is more sensitive in the detection of influenza A and B target analytes compared to commercially available influenza assays, while compared to PCR with only 1-2 The logarithm is less sensitive.

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Abstract

The present invention is directed to methods and apparatus for detection of one or more analytes. Analytes include agents or components of infectious agents such as pathogenic virus, as well as enzymes, proteins and biomarkers.

Description

[0001] priority [0002] This application claims priority to U.S. Provisional Application 61 / 177,272, filed May 11, 2009, and U.S. Provisional Application 61 / 228,135, filed July 23, 2009, each of which is hereby incorporated by reference. This article. [0003] Statement Regarding Government-Supported Research [0004] Portions of this invention were made with United States Government support under Contract No. 200-2007-19345 awarded by the Center for Disease Control. The government may have certain rights in portions of this invention. Background of the invention [0005] The present invention relates to assays for one or more analytes, eg, antigens, in a sample, eg, a biological sample obtained from a subject. In particular, the present invention relates to methods and devices for detecting one or more analytes using binding moieties that specifically target selected analytes. An analyte can be, for example, one or more infectious agents. [0006] Many types of assays h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/538G01N33/58C12Q1/68
CPCC12Q1/6804G01N33/558C12Q2565/625C12Q2525/113G01N33/54388
Inventor R·L·伊根G·P·利加德D·D·布克C·J·约翰逊A·贝伦基S·伍卡耶罗维驰J·齐斯S·卡斯塔南
Owner NEXUS DX
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