Reagent device for detecting helicobacter pylori antibody and method thereof

A technology of Helicobacter pylori and a detection method, which is applied in the field of clinical immunology detection, can solve the problems of poor accuracy and precision of detection results, cumbersome and complicated operation process, and inability to perform single-part detection.

A technology of Helicobacter pylori and a detection method, which is applied in the field of clinical immunology detection, can solve the problems of poor accuracy and precision of detection results, cumbersome and complicated operation process, and inability to perform single-part detection.

CN103185793BActive Publication Date: 2015-01-07SHENZHEN YHLO BIOTECH

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  • Reagent device for detecting helicobacter pylori antibody and method thereof
  • Reagent device for detecting helicobacter pylori antibody and method thereof
  • Reagent device for detecting helicobacter pylori antibody and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Example 1 Reagent device for detecting Helicobacter pylori antibody

[0097] Such as Figure 1-2 As shown, the reagent device for detecting Helicobacter pylori antibody described in the application of the present invention is in the shape of a strip, including a base body 10 with eight holes and a handle 20 positioned at one end of the base body 10, and the sequence of the eight holes is arranged from near the handle to Starting from the end 10, there are sample wells 11, auxiliary agent wells 12, enzyme conjugate wells 13, substrate wells 14, stop solution wells 15, diluent wells 16, reaction wells 17 and dilution wells 18. In the sample wells 11 The sample to be tested is contained, the auxiliary agent hole 12 is used for adding auxiliary reagents when detection is required (such as adding an adsorbent to the auxiliary agent hole when detecting IgA), the enzyme conjugate solution is added to the enzyme conjugate hole 13, and the substrate hole 14 is added. Substrate so...

Embodiment 2

[0100] Example 2 Reagent device 2 for detecting Helicobacter pylori antibody

[0101] Such as image 3 As shown, the basic structure of the reagent device for detecting Helicobacter pylori antibody in this embodiment is the same as that of the reagent device in Example 1. The reagent device also includes several support columns 50, and the support columns 50 are located at Under the substrate 10 in the reagent device, there is more than one hole between adjacent support pillars 50. The function of the support pillars 50 is to enhance the mechanical strength and balance of the substrate.

Embodiment 3

[0102] Example 3 Reagent device 3 for detecting Helicobacter pylori antibody

[0103] Such as Figure 4-6 As shown, it is a preferred embodiment of the reagent device for detecting Helicobacter pylori antibody described in the application of the present invention. Its basic structure is the same as that of Embodiment 1 or Embodiment 2, except that the reaction hole 17 is a detachable structure. 17 is made up of outer hole 171 and inner hole 172, and the bottom of outer hole 171 has bottom hole 174, and inner hole 172 passes through bottom hole 174 and closely fits with bottom hole 174, and stays between outer hole 171 and inner hole 172. Overflow chamber 173, the effect of overflow prevention chamber 173 is in the reaction process, if liquid overflows, can stay in the chamber, prevents from contaminating instruments and other reagent devices.

[0104] Further, the cooperating fixing method of the inner hole and the outer hole can also be designed as two sections of the outer ...

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Abstract

The invention discloses a reagent device for detecting a helicobacter pylori antibody and a method thereof; the reagent device is long strip-shaped, comprises a substrate with eight hole sites and a handle disposed at one end of the substrate; the eight hole sites comprise a sample hole, an adjuvant hole, an enzyme conjugate hole, a substrate hole, a stopping solution hole, a diluent hole, a reaction hole, and a dilution hole, which are arranged in sequence from the end near the handle. The reagent device of the invention realizes helicobacter pylori antibody detection with the device based on a principle of enzyme-linked immunoassay, is an independent, single-sample analytic detection method, and can be used cooperatively with corresponding specific analytical instruments; during detection, a detection reagent or a sample is injected by using a full automatic precision liquid filling device, which provides advantages of operation automation, accurate filling amount, high accuracy and precision of detection results; and the reagent device has wide application prospects.

Description

technical field [0001] The application of the present invention relates to a reagent device and a method for detecting Helicobacter pylori antibody, belonging to the technical field of clinical immunology detection. Background technique [0002] Helicobacter pylori (HP) is a spiral-shaped Gram-negative bacterium in the human gastric mucosa. The diameter of HP is about 0.85 μm, the length is about 2.9 μm, the bacteria are smooth, S-shaped, with 4 to 6 flagella, easy to adhere to the mucosa of the gastric antrum and gastric body near the pylorus, located in the deep layer of gastric mucus, Do not come into direct contact with stomach acid. HP is one of the common pathogenic bacteria in humans. It is transmitted from person to person through the oral route. Active HP can survive in river water for 1 week. It is an important cause of chronic gastritis, peptic ulcer and non-ulcer dyspepsia. Disease factor. [0003] The reason why Helicobacter pylori has attracted attention is ...

Claims

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Application Information

Patent Timeline
07 Jan 2015
Publication
CN103185793B
IPC
G01N33/569
Inventors
胡德明; 刘清波