Preparation method for anti-oxidation anti-ultraviolet active yeast extract based on low temperature plasma

A low-temperature plasma and plasma technology, which is applied in the application field of plasma technology, can solve the problems of difficulty in realizing mass production of LYCD, toxic effects on the human body, and complicated preparation methods, and achieves low production cost, low environmental pollution, and good treatment effect. Effect

Inactive Publication Date: 2013-07-17
PEKING UNIV
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Problems solved by technology

However, the traditional method is difficult to achieve mass production of LYCD, and its preparation method is cumbersome, time-consuming, laborious, and toxic to the human body.

Method used

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  • Preparation method for anti-oxidation anti-ultraviolet active yeast extract based on low temperature plasma
  • Preparation method for anti-oxidation anti-ultraviolet active yeast extract based on low temperature plasma

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Embodiment 1

[0044] 1. The night before, pick 0.5ml-1ml of the wild-type Saccharomyces cerevisiae cell 4741MAT a preservation bacteria solution, respectively in about 10ml, YPAD medium (glucose2%, with or without G418100ug / ml) overnight culture, make The detected OD reached about 1.5.

[0045] 2. According to the measured OD value, dilute the bacterial solution with fresh medium, the dilution ratio is (bacterial solution: fresh medium = 0.2: (OD-O.2)), then put it into a shaker and cultivate for one hour. At this time, the OD value of the bacterial solution will be about 1.0, which is the logarithmic growth phase.

[0046] 3. Take 50ml centrifuge tubes and add 4ml water to each centrifuge tube. After 1 hour, when the OD is about 0.5, take 3ml bacterial liquid from each strain, put it into a 5ml centrifuge tube and centrifuge at 1000rpm for 5min. Take out the centrifuge tube, discard the supernatant, resuspend the bacteria with 3ml of water, mix well and add to three 50ml centrifuge tubes...

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Abstract

The invention provides a preparation method for an anti-oxidation anti-ultraviolet active yeast extract by using an atmospheric pressure low temperature plasma generating device, belonging to the field of application of low temperature plasma technology. The preparation method is characterized by comprising the following successive steps: culturing yeast cells; selecting a type of an atmospheric pressure low temperature plasma generating device and setting working parameters; treating a suspension of the yeast cells by using atmospheric pressure low temperature plasma; and extracting active substances of the yeast cells having undergone plasma treatment. The preparation method provided by the invention has the advantage of capacity of highly efficiently and conveniently changing the activity of an enzyme in the yeast cells. The prepared active yeast extract can be used as an additive for articles of everyday use and has anti-ultraviolet, anti-oxidation, anti-ageing, fresh-keeping, sterilization and anti-inflammation effects and the like. According to the invention, since the active yeast extract is produced by using microbial fermentation, the environment and natural resources pose no influence, industrialization and automation can be easily realized, and the method has the advantages of low production cost, easiness, stability, easy regulation and control and a high success rate. Enterprises of various scales can invest on the preparation method provided by the invention.

Description

technical field [0001] The invention discloses a method for preparing an anti-oxidation and anti-ultraviolet yeast active extract by using an atmospheric pressure low-temperature plasma generator, and belongs to the application field of low-temperature plasma technology. Background technique [0002] Saccharomyces cerevisiae, also known as baker's yeast or budding yeast. Saccharomyces cerevisiae is the yeast most widely related to human beings. Traditionally, it is used to make food such as bread and steamed bread and brew wine. It is also the main raw material for making yeast extract, which is commonly used in culture media. As a eukaryotic model organism, its role is equivalent to that of the prokaryotic model organism Escherichia coli. Yeast is a single-celled microorganism, but it has the basic structure and function of eukaryotic cells. Yeast consists of cell wall, cell membrane, nucleus and cytoplasm. Among them, the protein content is 40%-60%, and its protein hydr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N9/08
Inventor 马若男封红青张珏方竞
Owner PEKING UNIV
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