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A recombinant plasmid containing a synthetic recombinant duck β-defensin-2 gene

A technology for recombining plasmids and defensins, which is applied in genetic engineering, plant gene improvement, recombinant DNA technology, etc., can solve the problems of complicated procedures, high cost, limitations, etc., and achieve the goal of increasing expression, facilitating maintenance, and high production efficiency Effect

Active Publication Date: 2016-06-29
佛山市广牧兴饲料有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the low content of natural defensins in animals, direct extraction is far from meeting the needs, and the cost is high, the yield is low, and the process is complicated; chemical synthesis is feasible but the cost is too high, and the application is limited; with the development of genetic engineering technology, through genetic recombination It is possible to produce defensin peptides with technology to meet the needs of research and production

Method used

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Examples

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Embodiment Construction

[0027] Now in conjunction with embodiment the present invention is described in further detail:

[0028] The duck β-defensin-2 (AvBD2) gene nucleotide sequence design and in vitro synthesis process of the present invention are realized in this way,

[0029] Referring to the cDNA gene sequence of the duck AvBD2 gene registered in GenBank, the nucleotide sequence of the mature peptide of the duck AvBD2 gene was modified according to the yeast codon preference, and then synthesized by gene synthesis (sent to Shanghai Sangong Bioengineering Technology Co., Ltd. gene synthesis), the nucleotide sequence of the duck β-defensin-2 (AvBD2) gene synthesized after transformation is as follows:

[0030] GATATGTTTTTGTGTAGAATTGGTTCTTGTCATTTTGGTAGATGTCCAATTCATTTGGTTAGAGTTGGTTCCTGTTTTGGTTTTAGATCTTGTTGTAAGTCTCCATGGGATGTTTAA.

[0031] The recombinant plasmid pPICZα-A-AvBD2 containing the gene of the present invention is realized in this way,

[0032] The construction process of the recombinant...

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Abstract

A recombinant plasmid containing a synthesized recombinant duck-defensin-2 gene. The construction process of the recombinant plasmid containing synthesized recombinant duck-defensin-2 gene is characterized by comprising steps of: (1) arrangement of an upstream primer and a downstream primer of the duck beta-defensin-2 gene; and (2) PCR amplification of gene fragments of the duck-defensin-2 gene: construction of a recombinant plasmid pPICZalpha-A-AvBD2. Compared with the prior art, the recombinant plasmid provided by the invention has antibacterial and growth promoting activity, is suitable for application to animal production, and has the advantages of low cost and high production efficiency.

Description

technical field [0001] The invention relates to a gene recombinant plasmid in the field of animal biomedical engineering. Background technique [0002] Animals used in modern animal husbandry are increasingly sensitive to environmental stress and pathogens while having high productivity. Especially under intensive feeding conditions, feeding animals face more stress and invasion of pathogens. There is an urgent need for a high-efficiency, non-toxic, non-residue immune enhancer and growth enhancer in animal husbandry production. While improving the disease resistance of feed animals, it can also improve the production performance of feed animals and the safety of animal products, so as to Promote the healthy development of animal husbandry and reduce the damage of animal husbandry to human health and the ecological environment. Previous studies have found that defensins have broad-spectrum bactericidal activity and super antimicrobial effect, and the mechanism of action is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/81C12N15/66C12N15/12
Inventor 张辉华韩小凤马保华毕英佐马静云谢青梅李辰
Owner 佛山市广牧兴饲料有限公司
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