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Application of arsenic trioxide in preparation of medicines for treating renal carcinoma

A technology of arsenic trioxide and anticancer drug, applied in the field of medicine, can solve problems such as hindering the expression of Akt

Inactive Publication Date: 2013-07-24
XINXIANG MEDICAL UNIV
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  • Abstract
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Problems solved by technology

In the study, we reported for the first time the effect of 0.5 μM arsenic trioxide on the PI3K-Akt signaling pathway in human renal cancer cell line 786-O cells. The results showed that 0.5 μM arsenic trioxide inhibited the proliferation of human renal cell line 786-O cells and also inhibited the expression of PI3K. The expression of mRNA and protein, which in turn hinders the expression of Akt

Method used

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Embodiment Construction

[0008] The following examples are used to illustrate the present invention, but not to limit the scope of the present invention. The effect of arsenic trioxide on the proliferation of human renal cancer cell 786-O and its PI3K-Akt transduction pathway.

[0009] 1 Materials and methods

[0010] 1.1 Cell culture

[0011] The renal clear cell carcinoma cell line 786-O was purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences, catalog number TCHu186. The purchased cells were inoculated in RPMI-1640 medium (10% fetal bovine serum and 50μg / mL penicillin), 5% CO2, incubator humidity 90%, 37°C for 3 days. Passage every 3 days of culture, and take the cells in logarithmic growth phase for experiment.

[0012] 1.2 Experiment grouping and design

[0013] The cells in the logarithmic growth phase were seeded in 96-well plates and cultured under 5% CO2. When 60% of the 96-well bottom area is covered with cells, aspirate the medium and replace it...

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Abstract

The invention discloses application of arsenic trioxide in preparation of medicines for treating renal carcinoma. The key point of the technical scheme is that arsenic trioxide inhibits proliferation of human renal carcinoma cells 786-O by inhibiting the PI3K-Akt transduction path, so that arsenic trioxide can be used for preparing medicines for treating renal carcinoma, and in particular for preparing medicines for treating renal clear cell carcinoma.

Description

Technical field [0001] The invention relates to the field of medicine, in particular to the application of arsenic trioxide in the preparation of drugs for treating kidney cancer. Background technique [0002] A large number of studies have shown that arsenic trioxide has the functions of inhibiting tumor cell proliferation and promoting cell apoptosis and has significant clinical effects. Studies have found that arsenic trioxide can inhibit the induction of cervical cancer cell apoptosis, and exert its effects by binding to cytokeratin 18 and down-regulating the expression of hTERT mRNA. Similarly, arsenic trioxide can inhibit the proliferation of human lymphocyte line T2. Studies have found that it may interfere with the proliferation of normal cells or tumor cells by regulating the functions of the SHP-1 promoter and CpG islands. In addition, arsenic trioxide can also inhibit the proliferation of liver cancer cells and promyelocytic leukemia cells. The most in-depth study is ...

Claims

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Application Information

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IPC IPC(8): A61K33/36A61P13/12A61P35/00
Inventor 张艳朱峰徐春阳李爽韩广业郭珂一尉娜姜洪波仲丽丽解娜杨纯生李静冯素萍刘旭代贝贝周永霞徐佳李卓俞刘志娟
Owner XINXIANG MEDICAL UNIV
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