Method for grafting pear polyploidy test-tube plantlet outside test tube

Abstract

A method for grafting a pear polyploidy test-tube plantlet outside a test tube includes the following steps that the pear polyploidy test-tube plantlet is cultured in a culture bottle filled with proliferation culture medium in a subculture multiplication mode, a mediate plantlet which is 1.5 meters tall is obtained, then the mediate plantlet is cultured in a culture bottle with a rooting medium to be strong, and a pear polyploidy scion plantlet is obtained; a pear seed is planted in a pot with nutrient soil, and a stock plantlet with a tender or semi-ligneous stem is obtained; in a green house, the pear polyploidy scion plantlet is grafted on the stock plantlet, and a pear polyploidy plantlet which is used for being planted in the field is obtained after 30 days of culture. Due to the fact that the pear polyploidy test-tube plantlet is cultured in advance, the stock plantlet is cultured in a field planting mode, the survival rate of the pear polyploidy plantlet is kept through a grafting method, the polyploidy test-tube plantlet is not directly cultured in a rooting mode, the field survival rate of the polyploidy plantlet is improved, and optimization of pear trees is accelerated.

Description

technical field [0001] The invention relates to a method for grafting and forming seedlings outside the test tube, in particular to a method for grafting and forming seedlings of pear tree polyploid test tube seedlings outside the test tube. Background technique [0002] Obtaining polyploid pear trees through artificial mutagenesis in the laboratory is an important method to obtain new varieties of pear trees and to breed pear trees. In order to successfully transplant the polyploid test-tube seedlings of pear trees in the laboratory to the field, Therefore the method that is used for the grafting of pear tree polyploid test-tube seedling outside the test tube is a kind of important fruit tree cultivation method, does not also have a kind of method that is used for the grafting of pear polyploid test-tube seedling outside the test tube now. Contents of the invention [0003] In order to overcome above-mentioned technical shortcoming, the purpose of this invention is to pro...

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Problems solved by technology

[0002] Obtaining polyploid pear trees through artificial mutagenesis in the laboratory is an important method to obtain new varieties of pear trees and to breed pear trees. In order to successfully transplant the polyploid test-tube seedlings of pear trees in the laboratory to the field, Therefore the method that is used for the grafting of pear tree polyploid test-tube seedlings outside the test tube is a kind of important fruit tree cultivation method, also does not have a kind of method that is used for the grafting of pear polyploid test-tube seedlings outside the test tubes and becomes seedlings now

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