Method for grafting pear tree polyploid test-tube seedlings out of test-tube into seedlings

Abstract

A method for grafting pear polyploid test-tube seedlings into seedlings outside the test tube, the steps are: carrying out subculture propagation and culturing of pear polyploid test-tube seedlings in a culture bottle equipped with a proliferation medium, and obtaining Middle green seedlings more than 1.5cm, then middle green seedlings are carried out strong seedling cultivation in the culture bottle that rooting medium is housed, obtain pear tree polyploid scion seedling; Plant pear seed in the pot that nutrient soil is housed, obtain The stem is tender or semi-lignified rootstock seedlings; the pear tree polyploid scion seedlings are grafted onto the rootstock seedlings in the greenhouse, and after 30 days of cultivation, the pear tree polyploid seedlings for planting in the field are obtained. Due to the design of pear tree polyploid test-tube seedlings for pre-cultivation, rootstock seedlings were planted and cultivated in the field, and the survival rate of pear polyploid seedlings was maintained by grafting, and the pear polyploid test-tube seedlings were no longer used directly Rooting cultivation, thus improving the field survival rate of polyploid seedlings of pear trees, accelerated the optimization of pear trees.

Description

technical field [0001] The invention relates to a method for grafting and forming seedlings outside the test tube, in particular to a method for grafting and forming seedlings of pear tree polyploid test tube seedlings outside the test tube. Background technique [0002] Obtaining polyploid pear trees through artificial mutagenesis in the laboratory is an important method to obtain new varieties of pear trees and to breed pear trees. In order to successfully transplant the polyploid test-tube seedlings of pear trees in the laboratory to the field, Therefore the method that is used for the grafting of pear tree polyploid test-tube seedling outside the test tube is a kind of important fruit tree cultivation method, does not also have a kind of method that is used for the grafting of pear polyploid test-tube seedling outside the test tube now. Contents of the invention [0003] In order to overcome above-mentioned technical shortcoming, the purpose of this invention is to pro...

AI Technical Summary

Problems solved by technology

[0002] Obtaining polyploid pear trees through artificial mutagenesis in the laboratory is an important method to obtain new varieties of pear trees and to breed pear trees. In order to successfully transplant the polyploid test-tube seedlings of pear trees in the laboratory to the field, Therefore the method that is used for the grafting of pear tree polyploid test-tube seedlings outside the test tube is a kind of important fruit tree cultivation method, also does not have a kind of method that is used for the grafting of pear polyploid test-tube seedlings outside the test tubes and becomes seedlings now

Images