Air pressure control device and method for patch clamp
An air pressure control and patch clamp technology, applied in the field of patch clamp, can solve problems such as air pressure cannot be switched, affect sealing quality and efficiency, negative pressure cannot be stably continued, etc., to achieve the effect of easy control and simple device structure
Inactive Publication Date: 2015-03-11
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology
At present, it is usually carried out by manual suction at home and abroad, and there are mainly the following problems: the gas pressure value obtained by manual suction is based on empirical values, which cannot be accurately calculated; the negative pressure provided by manual suction cannot be stable and continuous, and the Cells are often adsorbed again due to insufficient negative pressure, which affects the quality and efficiency of sealing; the air pressure provided by artificial blowing cannot be quickly switched between positive and negative pressures
Method used
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[0014] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not constitute a conflict with each other.
[0015] refer to figure 1 , the present invention includes a linear drive mechanism, a syringe, a first solenoid valve 1 and a second solenoid valve 11, the linear drive mechanism is connected to the push rod 6 of the syringe, and the bottom of the syringe 5 of the syringe is connected to the input ends 2 of the two solenoid valves respectively through the air pipe 4 , 12 connected; wherein the ...
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The invention discloses an air pressure control device for a patch clamp. The air pressure control device comprises a linear driving mechanism, an injector, a first electromagnetic valve and a second electromagnetic valve, wherein the linear driving mechanism is connected with a push rod of the injector; the bottom of an injection barrel of the injector is communicated with the input ends of the two electromagnetic valves through air pipes; the output end of the first electromagnetic valve is in normal pressure; and the output end of the second electromagnetic valve is connected with a ventilating pipe of an electrode clamp connected with the patch clamp in a sealed manner. The invention also provides a method for performing air pressure control on a patch clamp sealed connection process by using the device. According to the air pressure control device and the air pressure control method, under the displacement matching between a switch of the electromagnetic valve and the push rod of the injector, more continuously stable positive and negative pressure is automatically supplied to the sealed connection of the patch clamp; quick switch for the air pressure between the positive pressure and the negative pressure can be realized; and the whole device is simple in structure and convenient to control.
Description
technical field [0001] The invention belongs to the field of patch clamp technology, and more specifically relates to a patch clamp air pressure control device and method, which are suitable for positive and negative pressure control and switching in the patch clamp Geo-sealing process. Background technique [0002] As a powerful tool for electrophysiological research, patch clamp technology is widely used in the measurement of cell membrane ion channel current, cell secretion, pharmacology, pathophysiology, neuroscience, brain science, cell biology and molecular biology research, and plant cell research. Physiological research, etc. [0003] When using the patch clamp technique for cell physiology experiments, it is necessary to form a geo-seal (high-resistance seal) between the glass microelectrode and the surface of the cell membrane before subsequent experiments can be performed. Geo-sealing is generally accomplished by controlling the gas pressure in the inner cavity o...
Claims
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IPC IPC(8): C12M1/36C12M1/34
Inventor 肖靓章强沈安文沈汉林饶文培谢青吴颖吴元喜
Owner HUAZHONG UNIV OF SCI & TECH