Whole blood quality control material and preparation method thereof

A quality control substance and blood quality technology, applied in the field of clinical testing, can solve the problems of platelet count interference, complex extraction process, high cost, etc., and achieve the effect of obvious size difference, simple manufacturing process, and reduced interference

Active Publication Date: 2013-10-02
NANJING PERLONG MEDICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of these methods are: the erythrocyte simulant after stable treatment with porcine red blood cells is too small in size, which interferes with the platelet count, thereby affecting the real platelet count; while the source of human red blood cells is very difficult and difficult to carry out. mass production
[0005] U.S. Patent US4198206 reports a method of directly using human platelets as a quality control after stabilization treatment, but the source of human platelets is very difficult, and it is not easy to carry out large-scale production
[0006] U.S. Patent US42338564 reports a kind of simulated human platelets after stabilizing porcine platelets. However, porcine platelets are easy to aggregate and the extraction process is complicated. If an instrument specially used to separate porcine blood components is used, the cost will be high.

Method used

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  • Whole blood quality control material and preparation method thereof
  • Whole blood quality control material and preparation method thereof
  • Whole blood quality control material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Anticoagulant: In 1L of anticoagulant, glucose is 32.4g, citric acid is 3.85g, sodium dihydrogen phosphate is 4.82g, and the balance is water.

[0035] PBS buffer: In 1L of PBS buffer, 4.5g of sodium chloride, 22.1g of disodium hydrogen phosphate, and 2.1g of sodium dihydrogen phosphate.

[0036] White blood cell fixative: formaldehyde: glutaraldehyde (V:V) = 1:1 mixed solution, the final concentration of formaldehyde and glutaraldehyde is 5%.

[0037] Red blood cell and platelet fixative: in 1L red blood cell and platelet fixative, ATP 2.5g, lactose 75g, formaldehyde 0.85ml, F-680.36g, citric acid 0.5g, trisodium citrate 14.2g, sodium azide 3.0g , and the rest is water.

Embodiment 2

[0039] Anticoagulant: In 1L of anticoagulant, ethylenediaminetetraacetic acid 28.4g, citric acid 1.6g, sodium dihydrogen phosphate 1g, and the balance is water.

[0040] PBS buffer: In 1L of PBS buffer, 4.5g of sodium chloride, 22.1g of disodium hydrogen phosphate, and 2.1g of sodium dihydrogen phosphate.

[0041] White blood cell fixative: paraformaldehyde: F-68 (V:V) = 1:1 mixed solution, the final concentration is 0.05%.

[0042] Red blood cell and platelet fixative: in 1L red blood cell and platelet fixative, adenine 2.5g, glucose 47.5g, paraformaldehyde 0.3g, F-680.2g, citric acid 0.3g, trisodium citrate 10.2g, azide Sodium 0.5g, the rest is water.

Embodiment 3

[0044] Anticoagulant: In 1L of anticoagulant, glucose is 65.4g, trisodium citrate is 9.6g, sodium dihydrogen phosphate is 5.2g, and the balance is water.

[0045] PBS buffer solution: In 1L of PBS buffer solution, 5.5 g of sodium chloride, 20.3 g of disodium hydrogen phosphate, and 3.1 g of sodium dihydrogen phosphate.

[0046] White blood cell fixative: formaldehyde: glutaraldehyde (V:V) = 1:1 mixed solution, the final concentration of formaldehyde and glutaraldehyde is 10%.

[0047] Red blood cell and platelet fixative: in 1L red blood cell and platelet fixative, ATP10g, lactose 110g, formaldehyde 2ml, F-684.0g, disodium hydrogen phosphate 3.0g, sodium dihydrogen phosphate 2.0g, sodium azide 4.0g, the rest for water.

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Abstract

The invention discloses a whole blood quality control material. The whole blood quality control material comprises three cell simulating materials, an anticoagulation agent, white cell stationary liquid, and red cell and blood platelet stationary liquid. The invention further discloses a preparation method of the whole blood quality control material. The whole blood quality control material is wide in raw material resources and simple in preparation process; three classes of white cells can be clearly identified on a three-class blood cell instrument; the size difference of red cells and blood platelets is obvious, and the red cells and the blood platelets are easy to identify by the instrument; interferences of the small red cells on the blood platelets are small and the storage life can reach 6 months; the use requirements of clinical quality control are met.

Description

technical field [0001] The invention relates to the field of clinical testing, in particular to a whole blood quality control substance and a preparation method thereof. Background technique [0002] Whole blood quality control substances and calibrators are used in clinical laboratory experiments and necessary measurement substances for the traceability of hematology analyzers. They serve as the only basis for indoor and outdoor quality control, evaluation, and accuracy of laboratory results for whole blood analyzers. Most of the whole blood quality controls at home and abroad use animal blood cells or polymer materials as substitutes, and are widely used in clinical tests. However, most of the whole blood quality control substances of domestic manufacturers can only provide one or two white blood cell subgroups; on the hematology analyzer, the histogram of the test results can only provide one or two peaks, which is difficult to achieve intuitively. The effect of leukocyt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N1/28
Inventor 尹小东管中来
Owner NANJING PERLONG MEDICAL EQUIP
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