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A kind of inoculation method and inoculation device of gene immunity vaccine

An inoculation device and gene technology, applied in the field of immunization, can solve the problems of complex operation of the bullet production method, difficult humoral immunization, and low antibody titer, and achieve excellent active immunization effects, good application prospects, and high antibody titer effects

Inactive Publication Date: 2016-09-07
成都安铂奥金生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The immune effect of high-voltage electric shock and subcutaneous injection is poor, it is difficult to induce humoral immunity, and the titer of antibody produced is low
The immune effect of the gene gun method is relatively good, but in the gene gun technology, the gold particles or plasmid DNA solution attached to the plasmid DNA need to pass through the cuticle of the skin and enter the epidermal cells, and the thickness of the cuticle of the animal skin varies depending on the age of the experimental animal, season, Injection sites and individuals vary greatly, making the immune results extremely unstable. In addition, the raw materials of gold particles for making bullets are expensive, and the bullet manufacturing method is complicated to operate, requiring special inert gas high-pressure equipment, and the cost is high

Method used

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  • A kind of inoculation method and inoculation device of gene immunity vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Inoculation method of the present invention

[0035] 1. Inoculation method

[0036] (1) Use an insulin syringe (equipped with a 30-gauge needle) to inject 50 microliters of the gene-immune vaccine into the footpad of the mouse hindlimb by intradermal injection. mm, the major axis is 10 mm.

[0037] (2) Apply an electric field using the inoculation device of the gene immune vaccine of the present invention. Specific operations: 1). Put the electrode plate into the electrode sleeve, the electrode plate is 5 mm wide and 10 mm long; 2). Add a little phosphate buffer solution to the 3) Attach the anode electrode plate to the picchu injected with plasmid DNA, and attach the cathode electrode plate to the dorsal side of the injected hind limb of the mouse; 4). Turn on the power supply, Adjust the current to 3mA, and turn off the power after 5 minutes of power on.

Embodiment 2

[0038] Embodiment 2 Inoculation method of the present invention

[0039] 1. Inoculation method

[0040] (1) Use a disposable 1.0ml syringe to inject 500 microliters of the gene-immune vaccine on the outside of the ear of New Zealand white rabbits by intradermal injection, and the piculum formed is rectangular in shape, 20 mm wide and 30 mm long.

[0041] (2) Apply an electric field using the inoculation device of the gene immune vaccine of the present invention. Specific operations: 1). Put the electrode plate into the electrode sleeve, the electrode plate is 20 mm wide and 30 mm long; 2). Add a little phosphate buffer solution to the Put the electrode sleeve on, so that the electrode sleeve is completely soaked; 3). Attach the anode electrode plate to the plasmid DNA injection skin outside the rabbit ear, and attach the cathode electrode plate to the inner skin of the rabbit ear; 4). Turn on the power supply, Adjust the current to 5 mA, and turn off the power after 10 minute...

Embodiment 3

[0042] Embodiment 3 inoculation method of the present invention

[0043] 1. Inoculation method

[0044] (1) Use an insulin syringe (equipped with a 27-gauge needle) to inject 50 microliters of the gene-immune vaccine into the footpad of the mouse hindlimb by intradermal injection. mm, the major axis is 10 mm.

[0045] (2) Apply an electric field using the inoculation device of the gene immune vaccine of the present invention. Specific operations: 1). Put the electrode plate into the electrode sleeve, the electrode plate is 5 mm wide and 10 mm long; 2). Add a little phosphate buffer solution to the 3) Attach the anode electrode plate to the picchu injected with plasmid DNA, and attach the cathode electrode plate to the dorsal side of the injected hind limb of the mouse; 4). Turn on the power supply, Adjust the current to 3 mA, and turn off the power after 30 minutes of power on.

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Abstract

The invention discloses an inoculation method of gene vaccine. The method includes a first step of injecting the gene vaccine into a gap between the epidermis of a living animal and the dermis of the animal in an intradermal mode and enabling a skin colliculus to be formed in the injection portion, and a second step of applying an electric field on the injection portion, attaching a positive pole plate to the surface of the skin colliculus and attaching the negative pole to the opposite side of the injection portion so that the gene vaccine can be promoted to enter epidermal cells. The invention further provides an inoculation device of the gene vaccine. When the gene vaccine is inoculated through the method, the immune effect is good, the titer of produced antibodies is high, the operation is simple and convenient to conduct, the success rate is high, the repeatability is good, and the application prospect is good.

Description

technical field [0001] The invention relates to an inoculation method of a gene immune vaccine, that is, an inoculation device, belonging to the field of immunization. Background technique [0002] Gene immunization, also known as DNA immunization, nucleic acid immunization or genetic immunization, is a new immunological technology developed in the early 1990s. It recombines a foreign gene expressing a certain antigenic protein with a plasmid, and directly transfers the naked plasmid DNA into Live animal cells, and then express exogenous recombinant protein antigens in animals to stimulate strong and sustained humoral immune responses and cellular immune responses. [0003] There are many ways to inoculate genetic vaccines (plasmid DNA), mainly including high-voltage electric shock, subcutaneous injection and gene gun. [0004] The immune effects of high-voltage electric shock and subcutaneous injection are poor, it is difficult to induce humoral immunity, and the titer of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D7/00A61N1/30A61N1/04A61M5/00
Inventor 陈柏华韩星
Owner 成都安铂奥金生物科技有限公司