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A rapid detection method for sodium saccharin in wine
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A detection method, the technology of sodium saccharin, applied in Raman scattering, material excitation analysis, etc., can solve the problems of complicated and time-consuming operation, long detection time, high detection cost, etc., and achieve low detection cost, fast detection speed and rapid detection Effect
Inactive Publication Date: 2016-01-20
XIAMEN UNIV
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Problems solved by technology
These detection methods require complex pre-treatment of wine products, cumbersome and time-consuming operations, and require certain professional skills of testing personnel. The detection cost is also high and the detection time is long, which is not conducive to popularization in front-line supervision departments.
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Embodiment 155
[0029] The preparation of embodiment 155nmAu
[0030] Take 200mL mass fraction of 0.01% chloroauric acid aqueous solution in a 250mL round-bottomed flask, heat to boiling under reflux under magnetic stirring, then quickly add 1.4mL mass fraction of 1% sodium citrate aqueous solution, the solution changes within half a minute black, continue to reflux heating for 40min, the solution gradually changes from light yellow to brownish red, let it completely react and then naturally cool to room temperature to obtain a gold nanoparticle sol with a diameter of about 55 ± 10nm.
Embodiment 2
[0032] Preparation of sodium saccharin standard solution:
[0033] Take 0.1000g of sodium saccharin (manufacturer, purity), dissolve it, and set the volume in a 100ml volumetric flask. This is 1000ppm sodium saccharin standard solution.
[0034] Preparation of sodium saccharin standard solution: According to the previously prepared 1000ppm sodium saccharin standard solution, prepare 100ppm, 50ppm, 10ppm, 1ppm, 100ppb sodium saccharin standard solution.
[0035] Centrifugal concentration of 55nmAu (Citratesodium):
[0036] Sonicate 55nm Au for 5min, mix well, take 1.5ml of the sol in a 1.5ml plastic centrifuge tube, and centrifuge (8000rmp / min, 5min). Remove the supernatant and keep about 10ul of the sol.
Embodiment 3
[0037] Sodium saccharin detection in embodiment 3 liquor
[0038] A certain brand of white wine purchased in the market has been tested by other methods to contain no sodium saccharin
[0039] Set up a control group (blank)
[0040] 1. Sodium saccharin group (add sodium saccharin solution to blank liquor to make it contain 1ppm sodium saccharin)
[0042] 2. Use a 200ul pipette gun (manufacturer) to extract 200ul of the test solution and mix it with 10ul of 55nm Au (the color of the sol turns black) and place it in a detachable microtiter plate. Focus the laser focus on the surface of the object to be measured, and then start to collect spectra. The time for collecting spectra is 1s, the number of averages is 1, and the number of accumulations is 1. (Note: This process should be quick).
[0043] Note: The entire operation above was carried out at room temperature.
[0044] See t...
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Abstract
The invention discloses a rapid detection method for sodium saccharin in a wine product. The method comprises the following steps: (1) preparing a nanometer particle for Raman detection; (2) preparing standard sodium saccharin liquids with different concentrations; (3) respectively mixing the nanometer particle for Raman detection with the standard sodium saccharin liquids with different concentrations and subjecting obtained mixtures to Raman spectrum detection on a Raman spectrometer so as to obtain SERS spectra of sodium saccharin with different concentrations; (4) calculating characteristic peak intensity of the SERS spectra of the sodium saccharin with different concentrations so as to obtain an SERS intensity-concentration relation curve of the sodium saccharin; and (5) subjecting a to-be-detected wine liquid to Raman spectrum detection, comparing the characteristic peak of an obtained SERS spectrum of the to-be-detected wine liquid with the curve obtained in step (4) and determining whether the to-be-detected wine liquid contains sodium saccharin. The method provided by the invention has the advantages of a fast detection speed, low detection cost, no requirements on professional skills of detection personnel, immediate realization of rapid detection, etc.
Description
technical field [0001] The invention relates to a rapid detection method for sodium saccharin in wine products. Background technique [0002] Sweetener is an important class of food additives, it endows food with sweetness, improves the quality of food, has the advantages of low calorie, suitable for people with diabetes and so on. Sweeteners can be divided into natural sweeteners and artificial sweeteners according to their sources. The synthetic sweeteners widely used in the modern food industry mainly include sodium saccharin, cyclamate, aspartame, acesulfame potassium, sucralose, alitame and neotame. Among them, sodium saccharin is the oldest sweetener. Saccharin was discovered by American scientists in 1878 and was quickly accepted by the food industry and consumers. The chemical name of saccharin is o-benzoylsulfonimide, and the commercial saccharin sold in the market is actually a soluble sodium salt of o-benzoylsulfonimide, referred to as sodium saccharin. The sw...
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