Culture method of rice anther calluses

A culture method and callus technology, which is applied in the field of rice anther callus culture, can solve the problems of high labor intensity, anther damage, cumbersome operation, etc., and achieve the effects of relieving eye fatigue, improving collection speed, and avoiding contact pollution

Inactive Publication Date: 2013-10-23
无锡哈勃生物种业技术研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following problems: (1) The operation is cumbersome and difficult, labor-intensive, labor-intensive and time-consuming, and it is not easy to carry out large-scale production operations; Contact with anthers or medium can easily cause contact pollution, introduce bacteria, contaminate the medium, and also easily cause damage to anthers, affecting the callus induction rate
[0006] In addition to the difficulty of collecting rice anthers, in the in vitro culture of rice anthers, there is also the problem of low callus induction rate, and the induction medium is the key factor for callus formation. A lot of research has been done, such as Xiang Xunchao, Zhang Kaizheng, etc. (Xiang Xunchao, Zhang Kaizheng, etc., two factors that affect the anther culture effect of rice indica and japonica subspecies hybridization to construct backcross inbred lines. Plant Physiology Communications, 2006, 42(6):1041-1044) discusses the N 6 、SK 3 The influence of universal medium on the anther callus rate, the callus callus rate is about 8%, which is still relatively low

Method used

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  • Culture method of rice anther calluses
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Examples

Experimental program
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Effect test

Embodiment 1

[0034] (1) The flag leaf from the rice bract is pillowed to the second leaf, and when the glume of the rice panicle appears light green, the panicle is picked in the field, and the rice panicle is wrapped with moist gauze and pretreated at a low temperature of 4°C for 5 days.

[0035] (2) Put the treated rice ears in a sterile tissue culture bottle, disinfect the surface with 70% alcohol for 1 min, pour off the alcohol, disinfect with 1% sodium hypochlorite in a single-person sterile ultra-clean workbench for 15 min, pour Remove sodium hypochlorite and rinse 5 times with sterile water.

[0036] (3) Use scissors in a single-person sterile ultra-clean bench to cut off more than 1 / 3 of the top part of the glume that is born in the middle part of the panicle in a better physiological state, figure 1 The chaff 6 is shown intact, with the top chaff 7 removed.

[0037] (4) Under aseptic conditions, fill the bottom layer of the collection bottle into the culture medium, tighten the u...

Embodiment 2

[0042] (1) The flag leaf from the rice bract is pillowed to the second leaf, and when the glume of the rice panicle appears light green, the panicle is picked in the field, and the rice panicle is wrapped with moist gauze and pretreated at a low temperature of 4°C for 6 days.

[0043] (2) Put the treated rice ears in a sterile tissue culture bottle, disinfect the surface with 70% alcohol for 1 min, pour off the alcohol, disinfect with 1% sodium hypochlorite in a single-person sterile ultra-clean workbench for 15 min, pour Remove sodium hypochlorite and rinse 5 times with sterile water.

[0044] (3) Use scissors in a single-person aseptic ultra-clean bench to cut off more than 1 / 3 of the top part of the glume that is born in the middle part of the panicle and is in a better physiological state.

[0045] (4) Under aseptic conditions, fill the bottom layer of the collection bottle into the culture medium, tighten the upper and lower layers of the collection bottle, place the ears...

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Abstract

The invention discloses a culture method of rice anther calluses. The culture method comprises a step of disinfecting a rice ear, and removing tops of glumes; a step of suspending the rice ear, with the rice stalk upward and the rice ear downward, in a receiving flask containing nutrient solution to perform oscillation processing; a step of transferring the receiving flask after the oscillation processing is finished and performing induction culture in the absence of light to obtain the rice anther calluses. A formula of the nutrient solution is that an SK3 basic medium contains 2.5-3 g/L of proline, 0.4-0.6 g/L of glutamic acid, 1.8-2.5 mg/L of 2-4D and 1.8-2.5 mg/L of NAA. The culture method provided by the invention has advantages of anther collection convenience, high collection efficiency, and high inductivity and reduced pollution risk of the calluses.

Description

technical field [0001] The invention relates to the technical field of in vitro culture of plant anthers, in particular to a method for culturing rice anther callus. Background technique [0002] Anther culture is to use plant tissue culture technology to inoculate anthers that have developed to a certain stage on artificial medium through aseptic technique, so as to change the developmental program of pollen grains in anthers, induce their differentiation, and continuously undergo mitosis to form Cell clusters, and then form a mass of undifferentiated parenchyma-callus, or differentiate into embryoid bodies, and then induce callus to differentiate into complete plants. [0003] In my country, the rice anther culture technology is mainly used in three aspects: first, the combination of the flower culture technology (anther culture technology) and the hybrid breeding between conventional varieties for the cultivation of new varieties has achieved remarkable results. Second, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 汪庆冯都华邓丽傅俊杰舒庆尧崔海瑞黄建中吴忠明孙晓强吕晓亮
Owner 无锡哈勃生物种业技术研究院有限公司
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