High-space-resolution double-shaft confocal atlas micro-imaging method and device

A high-spatial-resolution, micro-imaging technology, applied in the field of spectral measurement, can solve the problems of insufficient spatial resolution, unfavorable light source intensity fluctuation suppression, and low collection efficiency, so as to improve the detection ability of micro-area Raman spectroscopy and improve the lateral resolution Force, improve the effect of measurement accuracy

Active Publication Date: 2013-11-27
BEIJING INSTITUTE OF TECHNOLOGYGY
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Problems solved by technology

[0009] The purpose of the present invention is to overcome the defects of low collection efficiency, insufficient spatial resolution, and unfavorable suppression of factors such as ambient backgr

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  • High-space-resolution double-shaft confocal atlas micro-imaging method and device
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  • High-space-resolution double-shaft confocal atlas micro-imaging method and device

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Embodiment

[0043] In this embodiment, the light source 1 is a laser, the polarization modulation system 29 is a radially polarized light generator, the dichroic light splitting system 8 is a Notch filter, the data processing system 21 is a computer, and the image acquisition system 11 is a CCD detector. 24 is a CCD detector, and the image magnification system 10 is a magnifying objective lens.

[0044] Such as figure 1 , figure 2 and Figure 5 As shown, the illumination objective lens 2 and the collection objective lens 7 are symmetrically distributed on both sides of the normal line 5 of the measurement surface, and the included angle between the illumination optical axis 4 and the normal line 5 of the measurement surface is θ 1 6. The angle between the collection optical axis 20 and the normal line 5 of the measurement surface is θ 2 31, where θ 1 = θ 2 , a high spatial resolution dual-axis confocal atlas microscopic imaging method, the measurement steps are:

[0045] Firstly, t...

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Abstract

The invention belongs to the technical field of spectral measurement and relates to a high-space-resolution double-shaft confocal atlas micro-imaging method and device. According to a core concept of the method, a double-shaft confocal micro-technique and a spectral detection technique are organically merged, and a normally-forgotten Reyleigh light is utilized to carry out auxiliary detection, so that the space resolution of a system is improved, and three detection modes including three-dimensional tomographic imaging, spectral detection and microcell atlas tomographic imaging are realized. The method and the device have the advantages of high space resolution, accuracy in positioning, high spectral detection sensitivity and the like, have wide application prospects in the fields of biomedicines, physical material science, petrochemical engineering, environmental science and the like and provide new ways for the high-space-resolution detection of microcell three-dimensional geometric positions and spectra.

Description

technical field [0001] The invention belongs to the technical field of spectrum measurement, and relates to a high spatial resolution dual-axis confocal atlas microscopic imaging method and device, which can be used for three-dimensional shape reconstruction and micro-region spectral detection of various samples. technical background [0002] Laser confocal Raman spectroscopy is an extremely important basic method for analyzing the structure and composition of samples. This technology not only inherits the high-resolution tomography features of confocal microscopy, but also can perform spectral analysis on samples, thus making laser confocal Raman spectroscopy unique in the field of spectral testing, and rapidly developing into an extremely important It is an important method for sample structure and composition analysis, making it widely used in frontier basic research in chemistry, biology, medicine, physics, geology, forensic evidence collection, criminal investigation an...

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Application Information

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IPC IPC(8): G01N21/65
Inventor 赵维谦王允崔晗邱丽荣
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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