Acremonium jx524288 and its application
A technology of JX524288, Acremonium peach, applied in the field of microorganisms, can solve problems such as unreported, achieve broad application prospects, promote germination, and overcome the effects of harm
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Embodiment 1
[0039] This example illustrates the isolation of strains.
[0040] Select a representative site on the hillside of the campus, use a shovel to dig a soil sample with a soil depth of about 4-10cm, put it into a sampling paper bag, the soil in the paper bag should have no large particles, mark the paper bag and bring it back to the experiment room for the isolation of fungi in the soil. Weigh 5g of the shade-dried soil sample, put it into a sterilized mortar and grind it until the soil sample is ground into particles of uniform size. Dilute the soil sample with sterile water to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 times the soil suspension, absorb about 0.1mL of soil suspension with different dilutions and spread it on the PDA medium, set three replicates for each group, and cultivate in the dark at 28°C incubator. After the colonies grow out, single colonies with different sizes, shapes and colors are picked and recultivated and purified on PDA plates.
[0041] Wherein, the p...
Embodiment 2
[0045] This example illustrates the determination of the antibacterial spectrum of bacterial strains.
[0046] According to the effective strains obtained in Example 1 with strong antagonistic ability against Rhizoctonia, the antibacterial spectrum was determined by the mycelial growth inhibition method. Mycelia growth inhibition method: Transfer various plant pathogenic bacteria stored in the laboratory refrigerator to PDA plates for activation, culture them in a 28°C incubator for 4-6 days, and use a sterile puncher with a diameter of 5mm to separate the pathogenic bacteria. Cut the bacteria at the edge of the bacteria into uniform size bacteria, use the inoculation needle to pick the bacteria and insert them into the PDA plate respectively, insert the bacteria that are antagonistic to the fungus at a distance of 3mm from the bacteria, repeat 3 times for each group, and place at 28°C The antibacterial radius between the antagonistic fungi and different pathogenic bacteria wa...
Embodiment 3
[0052] This example illustrates the cultivation of this strain.
[0053] Chase culture medium (recipe: 3g sodium nitrate, 1g dipotassium hydrogen phosphate, magnesium sulfate (MgSO 4 ·7H 2 O) 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, agar 15g, distilled water 1000mL) as the basic medium, weigh 20g of glucose, sucrose, lactose, maltose, soluble starch, mannose and cellulose to replace the check Sucrose in Shi's medium was used to make medium containing different carbon sources. Similarly, weigh 3g of urea, sodium nitrate, ammonium sulfate, peptone, soybean peptone, tryptone, and yeast extract to replace NaNO in Chase medium. 3 , made of medium containing different nitrogen sources.
[0054] Use 1mol / L hydrochloric acid and sodium hydroxide solution to adjust the pH value of the culture solution to 4.0, 5.0, 6.0, 7.0, 8.0, 8.5, 9.0 respectively, then add agar powder, pack and sterilize (121°C, 20min), Prepare media with different pH values. They were cultured in...
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