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Immunochromatographic test paper quickly detecting fleroxacin and preparation method of immunochromatographic test paper

A technology of immunochromatographic test paper and fleroxacin, which is applied in the field of immunochemical detection, can solve the problems of inability to carry out on-site detection in large-scale batch detection, affect the accuracy of analysis results, and require high professional quality, so as to protect the health of consumers and save Expenses for expensive instruments and equipment, and the effect of ensuring food safety

Active Publication Date: 2013-12-11
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the domestic and foreign methods for detecting fleroxacin residues mainly use physical and chemical analysis methods, including high performance liquid chromatography (HPLC), liquid chromatography / mass spectrometry (LC / MS), gas chromatography / mass spectrometry (GC / MS), etc., these methods are very effective, accurate and sensitive, but there are also certain defects, such as the complex pretreatment process of the samples to be tested, cumbersome and time-consuming; expensive instruments and equipment are required, and the promotion and use are limited; the quality of professionals is limited. High requirements; instrument maintenance requirements are high, and the quality of maintenance directly affects the accuracy of analysis results; high testing costs, on-site testing and large-scale batch testing are impossible, and timeliness is poor

Method used

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  • Immunochromatographic test paper quickly detecting fleroxacin and preparation method of immunochromatographic test paper

Examples

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Effect test

Embodiment 1

[0032] Example 1: Immunochromatographic test paper and preparation method for rapid detection of trace amounts of fleroxacin, see figure 1 , figure 2 . In the figure, the support layer 1 is made of plastic sheet strips, the absorbent fiber layer 2 is made of glass fiber cotton, the gold-labeled antibody fiber layer 3 is adsorbed with anti-floxacin monoclonal antibody, and the cellulose membrane layer 4 is made of nitrocellulose membrane. , The water-absorbing material layer 5 is made of water-absorbing filter paper, and the layers numbered 2, 3, 4, and 5 are pasted and fixed on the support layer 1 in sequence from right to left, and the fibers at the intersections cross and penetrate each other. On the cellulose membrane layer 4, there are detection blots 6 and control blots 7, the detection blots are printed with bovine serum albumin (BSA) solution coupled with fleroxacin, and the control blots are printed with goat anti-mouse IgG antibody solution , the two imprints are a...

Embodiment 2

[0047] Example 2: The immunochromatographic test paper is basically the same as Example 1, the difference is that the absorbent fiber layer is made of nylon membrane, the cellulose membrane layer is made of pure cellulose membrane, and both the detection blot and the invisible control blot are " Ten", the handle end protective film covering the water-absorbing material layer is blue.

[0048] The sample to be tested is chicken liver, which can be cut into pieces, smashed, and then centrifuged at 4000r / min for 5min, and the upper layer juice is taken as the sample to be tested;

[0049] Result judgment: (a) Positive If there is a brown-red blot on the cellulose membrane " ten ", indicating that the test result is positive, indicating that the sample to be tested contains fleroxacin; (b) negative if there are two brown-red blots on the cellulose membrane" ten ", indicating that the test result is negative, indicating that there is no fleroxacin in the sample to be tested; (c)...

Embodiment 3

[0050] Embodiment three: immunochromatography test paper is basically the same as embodiment one, and the difference is that the adsorption fiber layer is made of polyvinylidene fluoride membrane (PVDF), and the carrier protein solution coupled with fleroxacin is chicken ovalbumin ( OVA), the invisible control blotting band is made by blotting the goat anti-mouse IgG antibody solution on the cellulose membrane, the cellulose membrane layer is made of carboxylated cellulose membrane, and the handle end protective film covering the water-absorbing material layer is green, and the detection Both the blotting and the control blotting bands are "┬".

[0051] Judgment of results: (a) positive if there is a brown-red blot "┬" on the cellulose membrane, it means the test result is positive, indicating that the sample to be tested contains fleroxacin; (b) negative if it is on the cellulose membrane There are two brown-red bands "┬", indicating that the test result is negative, indicati...

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Abstract

The invention discloses immunochromatographic test paper quickly detecting fleroxacin and a preparation method of the immunochromatographic test paper. A bottom layer of the immunochromatographic test paper serves as a support layer; an intermediate layer serves as an adsorption layer; a protective layer is fixed on the adsorption layer; the adsorption layer sequentially comprises an adsorption fiber layer, a gold-labeled antibody fiber layer, a cellulose membrane layer and a water absorption material layer at a handle end from a test end; a detecting blot printed by a fleroxacin coupled carrier protein solution and a control blot goat printed by an anti-mouse IgG antibody solution are arranged on the cellulose membrane layer; and a gold-labeled antibody is a colloidal gold labeled fleroxacin monoclonal antibody. The immunochromatographic test paper is mainly used for quickly detecting fleroxacin in food, is simple in structure, strong in specificity, high in sensitivity and accuracy, easy and simple to operate, wide in application scope, low in price and easy to popularize and apply, and has better social and economic values.

Description

technical field [0001] The invention belongs to the field of immunochemical detection, and relates to an immunocolloidal gold immunochromatographic test paper for rapid detection of fleroxacin residues and a preparation method thereof. Background technique [0002] Fleroxacin (FLE), its chemical name is 6,8-difluoro-1-(2-fluoroethyl)1,4-dihydro-7-(4-methyl-1-piperazinyl)- 4-Oxo-3-quinolinecarboxylic acid, molecular formula C 17 h 18 f 3 N 3 o 3 , with a molecular weight of 369.34, is a broad-spectrum antibacterial drug, especially has strong antibacterial activity against Gram-negative bacteria, and has the advantages of less dosage and good antibacterial effect. However, it has certain toxic and side effects on the human body, mainly including gastrointestinal reactions, liver and kidney toxicity, and musculoskeletal side effects. The "2013 Animal and Animal Products Veterinary Drug Residue Monitoring Plan" formulated by the Ministry of Agriculture of my country clear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531G01N33/532
Inventor 胡骁飞张改平邓瑞广杨艳艳柴书军
Owner HENAN ACAD OF AGRI SCI
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